Adherence of Aggregatibacter actinomycetemcomitans via serotype-specific polysaccharide antigens in lipopolysaccharides.

Oral Microbiology and Immunology
O FujiseCasey Chen

Abstract

Gram-negative Aggregatibacter actinomycetemcomitans is recognized as an important periodontal pathogen. A striking property of this bacterium is its ability to form a tenacious biofilm adhering to abiotic surfaces. Both fimbrial and non-fimbrial adhesins are believed to be responsible for this ability. In our study, specific markerless mutants in the biosynthesis genes of cell surface polysaccharides were constructed with the Cre-loxP recombination system to identify non-fimbrial adhesin(s). Non-fimbriated A. actinomycetemcomitans strain ATCC29523 (serotype a) was used to construct a deletion mutant of serotype-a specific polysaccharide antigen (SPA-a) in lipopolysaccharide (LPS). The LPS was purified through a polymyxin B column following phenol extraction, and verified by silver staining following sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by immunoblot analysis using rabbit antisera raised against SPA-a. Strains were grown in broth for 2 days and examined for the adherence of bacterial cells on the glass surface. Strain ATCC29523 formed a thin film of bacterial growth on the glass surface. The deletion of SPA-a affected its ability to form this thin film. When this mutant was rescued with the wild-type SPA...Continue Reading

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Citations

Dec 2, 2009·Applied and Environmental Microbiology·Jeffrey J MargolisDenise M Monack
Feb 18, 2014·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Ya-An ChengChun-Pin Lin
Sep 3, 2011·Journal of Periodontal Research·A TanakaK Maeda
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Nov 9, 2019·Pathogens·Georgios N BelibasakisAnders Johansson
Dec 19, 2019·Periodontology 2000·Casey ChenJørgen Slots
Jan 27, 2015·Implant Dentistry·Enrico Felice GherlonePaolo Capparé

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