Adherence of Candida albicans to a fibrin-platelet matrix formed in vitro.

Infection and Immunity
P A Maisch, R A Calderone

Abstract

The adherence of Candida albicans to a fibrin-platelet matrix formed in vitro was studied. Platelet-rich plasma obtained from rabbits was incubated with thrombin and CaCl2 to form a clot in tissue culture dishes. Such clots were then infected with 3 x 10(7) C. albicans cells per 0.3 ml prelabeled with [U14C]-glucose, and the percent adherence was measured after 30 min of incubation by counting the radioactivity in saline washes of the clot as well as a streptokinase-streptodornase digest of the corresponding clot. Heat- and formaldehyde-killed cells did not adhere as well as viable cells. Pretreatment of C. albicans with trypsin, chymotrypsin, and pronase reduced adherence to the clots. Normal rabbit serum and anti-Candida antiserum also inhibited adherence 40 and 100%, respectively, Diethylaminoethyl-purified anti-Candida gamma globulin (1:8) completely inhibited adherence, whereas purified normal serum gamma globulin did not. Several Candida spp. and Saccharomyces cerevisiae showed differences in their ability to adhere to clots. C. albicans and C. stellatoidea presented the highest adherence, whereas C. krusei, C. guilliermondii, and S. cerevisiae adhered less readily. Other species were intermediate in their ability to adhere.

References

Mar 1, 1977·Infection and Immunity·E M Peterson, R A Calderone
Apr 1, 1978·Infection and Immunity·R A CalderoneM A Sande
Jul 1, 1978·Infection and Immunity·L H Kimura, N N Pearsall
Nov 1, 1973·Infection and Immunity·W F Liljemark, R J Gibbons

❮ Previous
Next ❯

Citations

May 1, 1988·Mycopathologia·L P SamaranayakeT MacFarlane
Jan 1, 1987·Critical Reviews in Microbiology·L J Douglas
Dec 11, 1981·Mycopathologia·L R Schwocho, R J Moon
Nov 11, 2003·Canadian Journal of Microbiology·Gary B DunphyIian Boomer
Jun 29, 2011·Journal of Applied Oral Science : Revista FOB·Adriana Gadotti MachadoCristiane Yumi Koga-Ito
Nov 10, 2013·Future Microbiology·Cornelia SpethGünter Rambach
Dec 19, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M E EllisW Ventura
Apr 14, 2004·Journal of Applied Microbiology·M A El-AziziN Khardori
Dec 17, 2009·Cellular and Molecular Life Sciences : CMLS·Michael R Yeaman
Jun 23, 2010·Antimicrobial Agents and Chemotherapy·Melphine M Harriott, Mairi C Noverr
Mar 10, 2010·Future Microbiology·Michael R Yeaman
Jan 1, 1987·Critical Reviews in Microbiology·F C Odds
Apr 1, 1993·Mycopathologia·R A Greenfield, W A Joyce
Jun 12, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Hélène VancraeynesteSamir Jawhara
Jan 20, 2000·Infection and Immunity·R RobertJ M Senet
Jul 6, 2014·Thrombosis and Haemostasis·Cornelia SpethCornelia Lass-Flörl
Feb 16, 2002·The Journal of Hospital Infection·H Giamarellou
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·W L ChaffinJ P Martínez
Jun 1, 1990·Infection and Immunity·J E CutlerM A Jutila
Jan 1, 1990·Journal of Clinical Microbiology·R J SherertzL L Fauerbach
Mar 1, 1993·Antimicrobial Agents and Chemotherapy·M R YeamanM A Ghannoum
Sep 1, 1996·Infection and Immunity·R RobertJ M Senet
Aug 1, 1984·Infection and Immunity·T J MarrieJ W Costerton
Jul 1, 1994·Antimicrobial Agents and Chemotherapy·M R YeamanA S Bayer
Oct 1, 1981·Infection and Immunity·S W AdlerW P Reed
Feb 1, 1987·Antimicrobial Agents and Chemotherapy·K M NugentL D Gray
Sep 1, 1985·Infection and Immunity·K J Kwon-ChungP T Magee
Mar 1, 1991·Microbiological Reviews·R A Calderone, P C Braun
Dec 1, 1981·Infection and Immunity·K G SkerlT Sreevalsan
Jan 1, 1986·Infection and Immunity·K A HobergR A Calderone

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.