PMID: 2510648Jul 1, 1989Paper

Adherence of monocytes to the aortic endothelium. Effect of the intraluminal pressure

Archives des maladies du coeur et des vaisseaux
A TedguiO Azogui

Abstract

It is now generally accepted that most of the foam cells formed in the early fatty streak arise from circulating monocyte-macrophages. The effect of transmural pressure on mononuclear cells adherence to the vascular endothelium has been studied in excised rabbit thoracic aorta. Mononuclear cells were obtained from blood by centrifugation through lymphocyte separation medium (Ficoll-Paque, specific gravity 1.077). The mononuclear cell layer was collected from the interface and washed twice in RPMI containing 10 p. 100 fetal calf serum to remove contaminating platelets. The mononuclear cells were radiolabeled by incubation for 60 min in 0.1 mCi/ml of 51-chromium. After incubation, the cells were washed twice to remove free chromium, and resuspended in 5 ml RPMI containing 10 p. 100 fetal calf serum, at a concentration of 2 x 10(6) cells/ml. After ligature of intercostal arteries, 2 segments from the descending thoracic aorta were excised at in vivo length and under physiological pressure in order to preserve endothelial integrity. Arterial segments were incubated in oxygenated Krebs solution, at 39 degrees C and the intraluminal solution was changed to the solution containing the labeled mononuclear cells. The pressure was establ...Continue Reading

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