Adhesion and colonization of Vibrio cholerae O1 on shrimp and crab carapaces

Journal of Food Protection
J Castro-Rosas, E F Escartín

Abstract

The potential of Vibrio cholerae O1 to attach to and colonize the carapaces of shrimp and crabs was evaluated. One million cells of V. cholerae O1 were spread within a circle on the external surfaces of separated carapaces and stored at 22 +/- 0.2 degrees C in a moist environment to permit adherence. Attached vibrios were counted directly by an immunofluorescence technique and by the pour plate technique after detachment of the cells. To study the colonization process, rifampicin-resistant strains of V. cholerae O1 were used. V. cholerae O1 strains, including those resistant to rifampicin, were able to attach to shrimp and crab carapaces. Dorsal crab carapaces showed higher levels of attachment than ventral carapaces. Colonization of V. cholerae O1 on these carapaces was also demonstrated. Both attachment and colonization on the shrimp exoskeleton were optimal at a salinity of 1.0 to 1.5%, a pH of 6.0 to 7.0, and a temperature of 37 degrees C. Less than 2% attachment at 3 degrees C contrasted with >20% attachment at 37 degrees C. Even at 3% NaCl, some attachment was observed. Although attachment percentages may appear low (2 to 20%), they represent significant numbers, about 3.7 to 5.6 log10 CFU per carapace. A rugose V. choler...Continue Reading

References

Jan 1, 1979·Applied and Environmental Microbiology·J KaperS W Joseph
Aug 1, 1979·Infection and Immunity·D R NalinL Cisneros
Sep 29, 1992·Lancet·E W RiceJ G Morris
Jun 1, 1990·Applied and Environmental Microbiology·M L TamplinR R Colwell
Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Mar 1, 1987·Applied and Environmental Microbiology·K AmakoA Umeda
Jan 1, 1983·Applied and Environmental Microbiology·A HuqR R Colwell
Feb 1, 1981·Applied and Environmental Microbiology·R R ColwellA Huq
Aug 1, 1993·Applied and Environmental Microbiology·C W Kaspar, M L Tamplin
Dec 1, 1996·The Journal of Infectious Diseases·J G MorrisJ A Johnson
Mar 2, 1999·Applied and Environmental Microbiology·R Tarsi, C Pruzzo
May 1, 1999·Applied and Environmental Microbiology·M O Elasri, R V Miller
Nov 17, 1999·Molecular Microbiology·P I Watnick, R Kolter
Oct 1, 1994·Journal of Food Protection·Susan A McCARTHY, Amy L Miller

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Citations

Aug 15, 2013·Letters in Applied Microbiology·C A Gómez-AldapaJ Castro-Rosas
Apr 7, 2004·Molecular Microbiology·Sudha Moorthy, Paula I Watnick
Sep 8, 2010·Journal of Food Protection·Javier Castro-RosasM del Refugio Torres-Vitela
Dec 24, 2013·Applied and Environmental Microbiology·Tiffany C WilliamsJames D Oliver
Nov 12, 2015·Biochemistry. Biokhimii︠a︡·E Yu MarkovS V Balakhonov
Oct 31, 2020·Frontiers in Cellular and Infection Microbiology·Ashok KumarNiraj Kumar
Feb 12, 2017·Microbiology Spectrum·Sailaja ChintagariMarlene Janes

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