Adhesion force measurements on functionalized microbeads: An in-depth comparison of computer controlled micropipette and fluidic force microscopy

Journal of Colloid and Interface Science
Tamás GerecseiRóbert Horváth

Abstract

Characterization of the binding of functionalized microparticles to surfaces with a specific chemistry sheds light on molecular scale interactions. Polymer or protein adsorption are often monitored by colloid particle deposition. Force measurements on microbeads by atomic force microscopy (AFM) or optical tweezers are standard methods in molecular biophysics, but typically have low throughput. Washing and centrifuge assays with (bio)chemically decorated microbeads provide better statistics, but only qualitative results without a calibrated binding force or energy value. In the present work we demonstrate that a computer controlled micropipette (CCMP) is a straightforward and high-throughput alternative to quantify the surface adhesion of functionalized microparticles. However, being an indirect force measurement technique, its in-depth comparison with a direct force measurement is a prerequisite of applications requiring calibrated adhesion force values. To this end, we attached polystyrene microbeads to a solid support by the avidin-biotin linkage. We measured the adhesion strength of the microbeads with both a specialized robotic fluid force microscope (FluidFM BOT) and CCMP. Furthermore, the bead-support contact zone was dir...Continue Reading

Citations

Sep 3, 2020·International Journal of Molecular Sciences·Haibo HuangLining Sun
Apr 4, 2021·Cells·Ashwini ShindeTuhin Subhra Santra
Jun 16, 2021·Journal of Colloid and Interface Science·Rita Ungai-SalánkiBálint Szabó
Sep 18, 2021·Scientific Reports·Rita Ungai-SalánkiRobert Horvath

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