PMID: 8604017Mar 1, 1996Paper

Adhesion mechanisms involved in C5a-induced eosinophil homotypic aggregation

Journal of Leukocyte Biology
M M TeixeiraP G Hellewell

Abstract

During the process of migration into tissues, leukocytes interact primarily with vascular endothelial cells but they have also been shown to interact with each other. In this study we investigated the adhesion mechanisms involved in guinea pig eosinophil homotypic aggregation as assessed by changes in light transmission. The anti-CD18 monoclonal antibody (mAb) 6.5E, at concentrations in excess of those previously shown to abrogate CD18-dependent eosinophil adherence to serum-coated plastic, inhibited C5a-induced eosinophil aggregation to a maximum of 49-68%. In contrast, the anti-intercellular adhesion molecule-1 (ICAM-1) mAb RR1/1, which binds to guinea pig eosinophils and has been shown to block guinea pig ICAM-1 function, had no effect on C5a-induced responses. Similarly, two functionally active anti-very late antigen-4 (VLA-4) mAbs has no effect on eosinophil aggregation and did not affect the CD18-independent component of the aggregation response. The role of L-selectin in eosinophil aggregation was investigated by using heparin, the selectin-binding polysaccharide fucoidin, and the anti-L-selectin mAb MEL-14. Heparin concentration dependently inhibited C5a- and platelet- activating factor- (PAF) induced aggregation but C5...Continue Reading

Citations

Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P ZoldhelyiJ T Willerson
Aug 29, 2013·ISRN Pharmacology·Clive Page
Mar 1, 1997·British Journal of Pharmacology·M M Teixeira, P G Hellewell
May 25, 2010·Journal of Thrombosis and Haemostasis : JTH·J L DreyfussH B Nader
Dec 17, 2015·Pharmacological Reviews·Barbara MulloyClive P Page
Aug 1, 1996·British Journal of Pharmacology·M M TeixeiraP G Hellewell
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne Burke-GaffneyPaul G Hellewell
Aug 5, 2017·Frontiers in Medicine·Miriam PeinhauptAkos Heinemann
Feb 13, 2001·British Journal of Pharmacology·M M TeixeiraP G Hellewell
Feb 22, 2011·The Journal of Biological Chemistry·Tomio YabeMakoto Kiso
Mar 17, 2000·Pulmonary Pharmacology & Therapeutics·Z Diamant, C P Page
Dec 28, 1999·European Journal of Pharmacology·M M TeixeiraP G Hellewell
May 1, 1997·Journal of Cardiothoracic and Vascular Anesthesia·D Royston
Mar 1, 2000·Biochemistry·S L FitzpatrickD M Waisman

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