PMID: 9434321Jan 22, 1998Paper

Adhesion molecule expression in vascular endothelial cells incubated with cancer cell line supernatant are inhibited by neutrophil elastase inhibitor (ONO-5046.Na)

Research Communications in Molecular Pathology and Pharmacology
F MatsumuraM Ogawa

Abstract

We investigated the role of a neutrophil elastase (NE) produced by cancer cell lines on intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells using two human non-small cell lung cancer cell lines, EBC-1 and PC-3. It is known that immuno-reactive NE is produced by EBC-1 cells, but not PC-3. In control study, Northern blot analysis revealed that in vitro ICAM-1 mRNA transcripts in the rat endothelial cell line (WK-5) was increased in the presence of purified NE while a specific neutrophil elastase inhibitor (ONO-5046.Na) inhibited ICAM-1 expression. Similarly, ICAM-1 mRNA levels in WK-5 cells were increased by the culture supernatant obtained from EBC-1, but not from PC-3. In addition, ONO-5046.Na inhibited the increase in ICAM-1 mRNA levels stimulated by immuno-reactive HE from EBC-1. These results suggest that EBC-1 cells produce immunoreactive NE which has biological function of NE to stimulate ICAM-1 expression in the endothelial cells.

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