PMID: 8971630Nov 1, 1996Paper

Adhesion of different Candida spp. to plastic: XTT formazan determinations

Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology
S Hawser

Abstract

Adhesion of synchronized yeast-phase Candida cells to tissue culture plastic was investigated using the tetrazolium salt, XTT. The procedure permits the direct enumeration of adherent yeasts following the metabolic conversion of the XTT tetrazolium salt, to its reduced formazan form, by mitochondrial dehydrogenases. Using this procedure, the formation of XTT formazan by Candida cells was typically related to the inoculum size. The adhesion of Candida yeast-phase cells from different Candida spp. to plastic was of the following order: C. krusei (n = 5) > C. albicans (n = 10) > C. glabrata (n = 6). Furthermore, preliminary experiments with several other species indicated that C. tropicalis (n = 2) may adhere as well as C. albicans and that one strain each of C. guilliermondii and C. parapsilosis appear to adhere to plastic in a similar fashion to C. glabrata. The data indicate the utility of the XTT tetrazolium based assay in enumerating the adhesion of different Candida spp. to plastic.

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Mar 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·S Hawser

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Citations

Aug 11, 2010·Archives of Microbiology·Chari Nithya, Shunmugiah Karutha Pandian
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Brian M PetersMairi C Noverr
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·G RamageJ L López-Ribot
Apr 12, 2005·Eukaryotic Cell·Gordon RamageJosé L López-Ribot
Aug 30, 2001·Journal of Clinical Microbiology·G RamageJ L López-Ribot
Jan 9, 2003·Journal of Clinical Microbiology·D M KuhnM A Ghannoum
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
Mar 2, 2011·Canadian Journal of Microbiology·K NeelofarLuqman A Khan
Apr 21, 2012·International Journal of Microbiology·Gordon RamageCraig Williams
Apr 14, 2012·Indian Journal of Pathology & Microbiology·P M PunithavathyThangam Menon
Nov 20, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C F RodriguesM Henriques
Jun 20, 2014·The Journal of Antimicrobial Chemotherapy·Laura Judith Marcos-ZambranoJesús Guinea
Oct 30, 2009·Critical Reviews in Microbiology·Gordon RamageJose Lopez-Ribot
Apr 14, 2004·Journal of Applied Microbiology·M A El-AziziN Khardori
Mar 1, 2019·Medical Mycology Journal·Tomoe IchikawaReiko Ikeda

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