Adhesion of human osteoblasts to titanium: A morpho-functional analysis with confocal microscopy

Micron : the International Research and Review Journal for Microscopy
Jacopo UggeriRita Gatti

Abstract

Properties of surface affect the interactions between the implant and osteoblasts and direct the clinical osteointegrative outcome. The aim of this in vitro study was to describe the adhesion of living human osteoblasts to titanium disks with differently prepared surfaces: sand blasted with ZrO(2) particles and acid-etched (Soft-SLA, S-SLA) or with Al(2)O(3) particles and acid-etched (Hard-SLA, H-SLA), smooth surface (SS). Confocal microscopy was exploited to follow cell morpho-functional features either on living cells (cell shape with calcein-acethoxymethylester and mitochondria with tetramethylrhodamine methyl ester) or on fixed cells (immunocytochemistry of beta1-integrin and of actin) 6h or 24h after seeding. The underlying surface was visualized simultaneously on the same field. No cytotoxic effect was detected at any time and on any surface. At 6h after seeding, osteoblasts showed either a rounded or polygonal shape on both rough surfaces. Several features suggested that adhesion was faster with a higher level of organization on S-SLA than on H-SLA. Indeed osteoblasts grown on S-SLA were wider and with more extended protrusions than those on H-SLA. Active mitochondria on S-SLA occupied perinuclear areas and cellular prol...Continue Reading

References

Apr 14, 1995·Science·E A Clark, J S Brugge
Aug 1, 1994·Journal of Biomedical Materials Research·J C KellerR Zaharias
Feb 1, 1997·Current Opinion in Cell Biology·K M Yamada, B Geiger
May 20, 1998·Clinical Oral Implants Research·A WennerbergS Danelli
Mar 18, 1999·Journal of Biomechanical Engineering·D M Brunette, B Chehroudi
Mar 11, 2000·Biomaterials·K Anselme
Aug 27, 2003·Journal of Dental Research·L PostiglioneG Rossi
Apr 8, 2004·Journal of Periodontology·Stefano GuizzardiRenato Scandroglio
Mar 2, 2005·Tissue Engineering·Cameron J WilsonMark J Pearcy
Jul 9, 2005·Biomaterials·Andrés J García
May 30, 2006·Biochimica Et Biophysica Acta·Vasiliki Anesti, Luca Scorrano
Jan 16, 2007·Micron : the International Research and Review Journal for Microscopy·R GattiS Guizzardi
Nov 21, 2007·Biomaterials·Isabella TognariniMaria Luisa Brandi
Dec 7, 2007·Journal of Periodontology·Jacopo UggeriRita Gatti

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Citations

Mar 13, 2012·Clinical Oral Investigations·Thomas ZiebartBilal Al-Nawas
Apr 13, 2012·Colloids and Surfaces. B, Biointerfaces·Yinli LiWael Mamdouh
Dec 27, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Wanlei YangYu Qian
Jun 19, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shaobing LiLei Zhou
Nov 3, 2010·Macromolecular Bioscience·Arnold W G NijhuisJohn A Jansen
Jan 13, 2015·Journal of Materials Science. Materials in Medicine·Claudia BergemannJens Fischer
Apr 19, 2016·Journal of Biomedical Materials Research. Part a·Katherine L SnyderRupak M Rajachar
Nov 30, 2016·The Journal of Oral Implantology·Miriam TingJon B Suzuki
Oct 8, 2017·Journal of Biomedical Materials Research. Part a·D V PortanG C Papanicolaou
Nov 14, 2020·International Journal of Molecular Sciences·Jörn SchaeskeAndreas Winkel
Sep 3, 2020·ACS Applied Materials & Interfaces·Camila B TovaniAna P Ramos

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