Adhesion of Lactobacillus species in urine and phosphate buffer to silicone rubber and glass under flow

Biomaterials
K W MillsapH J Busscher

Abstract

Coating uroepithelial cells or catheter materials with lactobacilli has been shown to retard the development of a uropathogenic biofilm, with biosurfactant production and strong adhesion being two prerequisite properties of the Lactobacillus strains to be employed. In this paper, adhesion of six selected Lactobacillus strains to silicone rubber and glass in urine and in a phosphate buffer was studied using a parallel plate flow chamber. In addition, adhesive cell surface properties of the lactobacilli, i.e. the pH dependences of their zeta potentials and their hydrophobicities by water contact angles, were determined. L. acidophilus ATCC 4356 and L. fermentum B54 were the only strains showing significant adhesion to both hydrophobic silicone rubber and hydrophilic glass, possibly by virtue of their high cell surface hydrophobicities (water contact angles of 68 and 75 degrees, respectively) and small zeta potentials (-10.0 and -8.1 mV in buffer, respectively). Both hydrophobic Lactobacillus strains adhered less well in urine than in buffer. The remaining Lactobacillus strains studied were hydrophilic, with water contact angles between 25 and 36 degrees, and had highly negative zeta potentials, reaching -37.7 mV in buffer. Adhesi...Continue Reading

References

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Citations

Sep 18, 2004·Journal of Bacteriology·Virginia Vadillo-RodríguezHenny C van der Mei
Feb 4, 2010·International Journal of Biomaterials·Cláudia SousaRosário Oliveira
Sep 15, 2014·Colloids and Surfaces. B, Biointerfaces·J M R MoreiraF J Mergulhão
Nov 13, 2008·Journal of Bioscience and Bioengineering·Hiromi KubotaHiroo Uchiyama
Dec 23, 2016·Applied Microbiology and Biotechnology·Magdalena Polak-BereckaAdam Waśko
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira
Aug 25, 2018·Bioinspiration & Biomimetics·Betina Lopez-MilaCesar Rodriguez-Emmenegger
Jul 27, 2006·Journal of the American Chemical Society·Varun SambhyAyusman Sen

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