Adhesion of vancomycin-resistant enterococcus to human intestinal mucus

Current Microbiology
Nicole J PultzCurtis J Donskey

Abstract

The intestinal mucus layer provides a potential niche for colonization by vancomycin-resistant Enterococcus faecium (VREF). We therefore examined the ability of six VREF strains to adhere to human intestinal mucus and determined binding kinetics. Four of six (67%) VREF strains demonstrated significant adhesion to immobilized intestinal mucus compared with a Salmonella typhimurium-negative control strain, but the level of adherence was low compared with Lactobacillus rhamnosus GG. Binding kinetics studies demonstrated that the maximum number of these four VREF strains that could adhere to a unit surface area of immobilized mucus was similar to or higher than the maximum number of L. rhamnosus GG that could adhere; however, L. rhamnosus GG demonstrated 20- to 130-times higher affinity than the VREF strains. These results demonstrate that VREF strains may adhere to human intestinal mucus and suggest that L. rhamnosus GG might be able to displace VREF strains.

References

Feb 1, 1983·Infection and Immunity·R FreterK E Carey
Jan 1, 1995·Methods in Enzymology·P S Cohen, D C Laux
Oct 26, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C J DonskeyL B Rice
Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·C AtumaL Holm
Jun 12, 2001·Methods in Enzymology·A C OuwehandS Salminen
Jul 11, 2003·Clinical and Diagnostic Laboratory Immunology·Arthur C OuwehandEeva Salminen
Nov 26, 2003·Veterinary Microbiology·Minna RinkinenArthur C Ouwehand
Dec 14, 2004·Journal of Microbiological Methods·Satu VesterlundArthur C Ouwehand
Dec 22, 2004·Veterinary Research Communications·A LaukováA Ouwehand

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