Adhesion regulation of stromal cell-derived factor-1 activation of ERK in lymphocytes by phosphatases.

The Journal of Biological Chemistry
Tonya Laakko, Rudolph L Juliano

Abstract

We have investigated whether chemokine signaling to the extracellular-signal-regulated kinase (ERK) was regulated by beta 1-integrin-mediated adhesion in B- and T-cell lines. Activation of ERK by the chemokine SDF-1 can be regulated by adhesion to beta 1-integrin substrates in the T-cell lines MOLT-3, Jurkat, and H9 and in the Daudi B-cell line. In Jurkat T-cells, adhesion to the immobilized alpha 4 beta 1-integrin ligand VCAM-1 or to the alpha 5 beta 1-integrin ligand fibronectin regulated stromal-cell derived factor-1 (SDF-1) activation of ERK. Adhesion control of SDF-1 signaling was a rapid event, occurring as early as 10 min after adhesion, and loss of signaling occurred within 10 min of deadhesion. In contrast, SDF-1 activation of the ERK kinase MEK was independent of adhesion. Partial restoration of signaling to ERK in suspension was accomplished by pretreatment with pharmacological inhibitors of serine/threonine or protein-tyrosine phosphatases. In addition, we used a non-radioactive phosphatase assay using phosphorylated ERK as the substrate to determine relative ERK dephosphorylation in whole cell extracts. These results showed greater relative ERK dephosphorylation in extracts from Jurkat cells treated in suspension, ...Continue Reading

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Citations

Jan 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rania Kairouz-WahbeErkki Ruoslahti
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Min Chul ParkSunghoon Kim
Sep 3, 2011·PloS One·Priya Prakash KarmaliHector Biliran
Apr 10, 2007·Experimental Cell Research·Masaru MitsushimaNoriyuki Kioka
Oct 7, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shailesh SinghJames W Lillard
Dec 8, 2004·The Journal of Biological Chemistry·David J Levinthal, Donald B Defranco
Jan 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Asya RollsMichal Schwartz
Dec 8, 2020·Journal of Autoimmunity·Asmi ChakrabortyCharles J Dimitroff

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