Adhesion-related glycocalyx study: quantitative approach with imaging-spectrum in the energy filtering transmission electron microscope (EFTEM)

FEBS Letters
M SolerC Foa

Abstract

Large polysaccharide molecules composing the glycocalyx have been shown to prevent cell adhesion. However, this process was not observed microscopically. Terbium labeling, combined with a new quantitative imaging method based on electron energy loss spectroscopy, allowed specific glycocalyx staining with excellent contrast. Image analysis enabled us to compare glycocalyx structure in free membrane areas and contacts between monocytic cells and bound erythrocytes. Apparent glycocalyx thickness, in contact areas, was half of the sum of glycocalyx thicknesses in free areas without label density increase. Ultrastructural immunogold localization of CD43 molecules, a major component of glycocalyx, was also demonstrated to be excluded from contact areas during adhesion. Thus, both approaches strongly suggest that some glycocalyx elements must exit from contact to allow binding of adhesion molecules.

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B ArdmanD E Staunton
Nov 21, 1991·Nature·Y RosensteinS J Burakoff
May 31, 1991·Biochemical and Biophysical Research Communications·Y J JinQ R Wang
Jan 1, 1991·Annual Review of Immunology·E A Clark, P J Lane
Aug 2, 1990·Nature·T A Springer
Jan 1, 1988·Ultramicroscopy·R D Leapman, R L Ornberg
Nov 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Griffiths, H Hoppeler
Jul 11, 1985·Biochimica Et Biophysica Acta·T OhyashikiT Mohri
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·I CarrJ Rutherford
Aug 14, 1967·Journal of Molecular Biology·R C Valentine, N M Green
Jan 1, 1968·Experimental Cell Research·B J Clarris, J R Fraser
May 1, 1983·European Journal of Immunology·P BongrandP Golstein
Jun 1, 1984·The Journal of Experimental Medicine·E Remold-O'DonnellF S Rosen
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Oct 12, 1995·Nature·N ManjunathB Ardman
Jul 1, 1996·Journal of Microscopy·A L BeckersH G Van Eijk

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Citations

Jan 5, 2002·European Journal of Pharmacology·Michał KurzelewskiAndrzej Beresewicz
Oct 18, 2013·American Journal of Physiology. Cell Physiology·Michael J Mitchell, Michael R King
Dec 17, 2009·PLoS Computational Biology·Matthew J PaszekDaniel A Hammer
Aug 4, 2012·Current Opinion in Cell Biology·David Boettiger
Mar 22, 2001·Biophysical Journal·K D RinkerG A Truskey
Apr 13, 2001·Microscopy Research and Technique·C QuintanaJ L Carrascosa
Jul 29, 2003·Anesthesiology·Akira Suzuki, David M Eckmann
Jul 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Alina A ConstantinescuJos A E Spaan
Nov 24, 2006·Anesthesiology·David M Eckmann, Stephen C Armstead
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Aug 25, 2016·Cellular and Molecular Bioengineering·Matthew A DragovichX Frank Zhang
Feb 13, 2010·European Journal of Medical Research·Klaus F Hofmann-KieferM Rehm
Apr 4, 2021·International Journal of Molecular Sciences·Stephan AltmannRegina Ebert
May 29, 2018·ACS Biomaterials Science & Engineering·Carolyn R ShurerMatthew J Paszek
Sep 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Debanjan BaruaRudolf Winklbauer

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