PMID: 15348349Sep 7, 2004Paper

Adhesion strength of individual human bone marrow cells to fibronectin. Integrin beta1-mediated adhesion

Journal of Materials Science. Materials in Medicine
George M Athanassiou, D D Deligianni

Abstract

The purpose of this work was to study the adhesion strength of individual bone marrow cells, using a micropipette aspiration technique. The adhesion strength of the primary human bone marrow cells to fibronectin-coated substrate, by blocking the beta1 integrin with and without antibodies, was also determined. Human bone marrow stromal cells of the second passage were seeded at a density of 500 cells/cm2 on two different substrates: plastic culture dish (PCD) and PCD coated with fibronectin. In short adhesion times (15-180 min) the cells attached without spreading and remained almost spherical. A negative pressure of about 3500 Pa was applied, through the micropipette, on individual bone marrow cells and the detach process was recorded. The tip of the micropipette was bent at an 130 degrees angle to the corpus of the pipette and it was manipulated to be on the upper side of the cell and vertically to the bottom of the plate. It was observed from the experiments that the cells exhibited smaller adhesion strength at early adhesion times (30-85 min). After 85 min the adhesion strength increased abruptly and remained relatively constant for the adhesion period from 85 to 180 min for all substrates. Monoclonal antibodies against inte...Continue Reading

Citations

Nov 26, 2010·Journal of Biomedical Materials Research. Part a·Asha Shekaran, Andrés J García
Apr 10, 2012·Journal of Materials Science. Materials in Medicine·Petros A KokkinosDespina D Deligianni
Mar 2, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Max J LermanJohn P Fisher
Feb 19, 2010·Tissue Engineering. Part B, Reviews·Lilia Araida Hidalgo-Bastida, Sarah H Cartmell
Mar 1, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Miklós WeszlZsombor Lacza
May 26, 2017·Cellular & Molecular Biology Letters·Edyta WrobelEdyta Brzoska
Oct 27, 2010·Annals of Biomedical Engineering·Antonia G Moutzouri, George M Athanassiou

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