Adhesion structures of amniotic membranes integrated into human corneas

Investigative Ophthalmology & Visual Science
Miklós D ReschBerthold Seitz

Abstract

The aim of this study was to investigate the structural relationship between integrated amniotic membrane (AM) and corneal tissues in various integration patterns, focusing on adhesion structures along the interface. Fourteen eyes of 14 patients (age, 65.8 +/- 13.5 years) underwent penetrating keratoplasty (PKP) 19.3 +/- 20.7 weeks after cryopreserved human AM transplantation (AMT). The corneal buttons (after PKP) and the corresponding original AM (before AMT) were examined with the use of transmission electron microscopy (TEM) and immunohistochemistry for integrin beta4, type VII collagen, and laminin. Main outcome measures included thickness of the corneal epithelium and AM, density of the epithelial desmosomes and hemidesmosomes, continuity, and thickness of the epithelial basement membrane. Integrated AM was found by slit lamp in only 2 of 14 patients, but histology and TEM revealed AM integration in 11 of 14 patients up to 77 weeks after AMT. No amniotic epithelial cell was detected in any cornea with integrated AM stroma. Three basic patterns of integration could be described: subepithelial, intraepithelial, and intrastromal. Hemidesmosomes anchored the corneal epithelial cells to the AM at a density up to 165.3 +/- 22.9 ...Continue Reading

Citations

Nov 22, 2007·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·B Seitz
Feb 5, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Miklós D ReschBerthold Seitz
Jun 28, 2011·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Miklós D ReschErzsébet Csányi
May 19, 2007·The British Journal of Ophthalmology·C CursiefenF E Kruse
May 6, 2009·Journal of Tissue Engineering and Regenerative Medicine·M González-AndradesM Alaminos
Mar 11, 2009·Journal of Tissue Engineering and Regenerative Medicine·E HadjipanayiV Mudera
Feb 12, 2011·American Journal of Ophthalmology·Mario NubilePaolo Carpineto
Dec 16, 2006·Current Eye Research·Joo Youn OhJin Hak Lee
Jul 1, 2017·Journal of Ophthalmology·Hua-Tao XieMing-Chang Zhang
Aug 18, 2020·Clinical Ophthalmology·Andrew Walkden
Jun 15, 2007·Current Opinion in Ophthalmology
Dec 1, 2019·Archivos de la Sociedad Española de Oftalmología·J Lacorzana

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