Adhesion to fibronectin or collagen I gel induces rapid, extensive, biosynthetic alterations in epithelial cells

Journal of Cellular Physiology
R M LafrenieK M Yamada

Abstract

Extracellular matrix influences many cellular events. In this study, we demonstrate that adhesion of human salivary gland (HSG) epithelial cells to fibronectin- or collagen I gel-coated substrates, mediated by beta1 integrins, results in substantial alterations in protein and RNA expression profiles. The large numbers of changes in expression suggest that simply changing the adhesive substrate has basic effects on the regulation of cellular biosynthesis. Two-dimensional electrophoresis of [35S]methionine-labeled HSG cell proteins identified significant differences in the patterns of protein expression by cells cultured on nonprecoated substrates, collagen I gels or fibronectin. Thirty-two differentially expressed cDNA clones, which included both novel and previously sequenced genes, were up-regulated within 6 hr by culturing HSG cells on fibronectin or collagen I gels. Therefore, adhesion to collagen I or fibronectin resulted in rapid, widespread changes in cellular biosynthetic control. Expression of some genes was induced by ligation of beta1 integrins with antifunctional antibodies, whereas the expression of other genes was not induced. Most of the differentially expressed genes were up-regulated by adhesion to both fibronec...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E SeftorM J Hendrix
Jan 1, 1990·The International Journal of Biochemistry·Y Kagawa, S Ohta
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L E Dike, S R Farmer
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C D RoskelleyM J Bissell
Mar 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D B Williams
Nov 1, 1995·The Journal of Cell Biology·S MiyamotoK M Yamada
Mar 1, 1995·Molecular Biology of the Cell·X Zhu, R K Assoian
Apr 14, 1995·Science·E A Clark, J S Brugge
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Richardson, J T Parsons
Jan 6, 1995·The Journal of Biological Chemistry·N MorinoY Nojima
Jan 1, 1994·Journal of Cell Science. Supplement·J T ParsonsC Otey
Apr 1, 1994·Molecular Biology of the Cell·M A Schwartz, D E Ingber
Jan 1, 1994·Cell Adhesion and Communication·N Koseki, K Yoshizato
May 20, 1994·Cell·E Ruoslahti, J C Reed
Jan 1, 1993·Methods in Enzymology·G Brady, N N Iscove
Oct 1, 1993·Journal of Cellular Physiology·H LarjavaJ Heino
Oct 1, 1995·Current Opinion in Cell Biology·K M Yamada, S Miyamoto
Oct 1, 1995·Current Opinion in Cell Biology·C D RoskelleyM J Bissell
Jun 15, 1996·Journal of Cellular Biochemistry·R M Lafrenie, K M Yamada
Apr 1, 1996·Cell Adhesion and Communication·N KosekiK Yoshizato

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Citations

May 26, 2004·Biomaterials·Catherine M Klapperich, Carolyn R Bertozzi
Apr 25, 2003·Journal of Virological Methods·Dzung C Thach, David A Stenger
May 26, 1998·Annals of the New York Academy of Sciences·R M Lafrenie, K M Yamada
Jan 26, 1999·Annals of the New York Academy of Sciences·S MiyamotoK M Yamada
Jul 23, 1999·Annals of the New York Academy of Sciences·B J BaumK M Yamada
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·S J Yarwood, J R Woodgett
May 29, 2003·The Journal of Cell Biology·Thomas ManesLucia R Languino
Feb 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Howard DangChih-Ko Yeh
May 4, 2004·Journal of the Society for Gynecologic Investigation·Clara M SalamancaNelly Auersperg
Nov 22, 2019·Frontiers in Cell and Developmental Biology·Danielle WuMary C Farach-Carson
Nov 16, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Eugenia Awuah BoadiBidhan C Bandyopadhyay

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