Adhesion to substrates induces dendritic cell endothelization and decreases immunological response.

Immunobiology
Jacob OsterburFabian Benencia

Abstract

Dendritic cells (DCs) are antigen presenting cells capable of inducing specific immune responses against microbial infections, transplant antigens, or tumors. DCs have been shown to possess a high plasticity showing different phenotypes in response to their microenvironment. For example, tumor-associated DCs can acquire an angiogenic phenotype thus promoting tumor growth. Further, DCs cultured in vitro under different conditions are able to upregulate the expression of endothelial markers and to express angiogenic factors. Indeed, it has been shown that soluble factors such as VEGF of PGE-2, that are present in the microenvironment of several tumors, affect the biology of these cells. We hypothesize that in addition to soluble factors the adhesion to different substrates will also define the phenotype and function of DCs. Herewith we demonstrate that murine myeloid(m) DCs upregulate endothelial markers such as VE-Cadherin, and to a lesser extent TIE-2, and decrease their immune capabilities when cultured on solid surfaces as compared with the same cells cultured on ultra-low binding (ULB) surfaces. On the other hand, the expression of angiogenic molecules at the level of RNA was not different among these cultures. In order to f...Continue Reading

References

May 1, 1991·European Journal of Immunology·R A ScheerenS T Pals
Jan 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Q Yu, B P Toole
Apr 7, 2000·Carcinogenesis·K F RobyP F Terranova
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Aug 10, 2000·Differentiation; Research in Biological Diversity·B Fernandez PujolK Havemann
Jun 19, 2001·Current Opinion in Immunology·A Lanzavecchia, F Sallusto
Apr 10, 2002·American Journal of Physiology. Cell Physiology·Michael Papetti, Ira M Herman
Aug 30, 2002·Trends in Immunology·Manfred B Lutz, Gerold Schuler
Sep 20, 2002·Skin Pharmacology and Applied Skin Physiology·J C SimonS Martin
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Helmut PontaPeter A Herrlich
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Jun 25, 2003·Biochemical and Biophysical Research Communications·Masafumi NakayamaHideo Yagita
Oct 24, 2003·Cell and Tissue Research·Valentin DjonovPeter H Burri
Mar 27, 2004·The Journal of Biological Chemistry·Svetlana Gingis-VelitskiNeta Ilan
Jul 6, 2004·Immunological Reviews·Eli Gilboa, Johannes Vieweg
Aug 6, 2004·Endocrine Reviews·Napoleone Ferrara
Aug 13, 2004·Atherosclerosis·Atilla YilmazChristoph D Garlichs
Aug 18, 2004·Cancer Research·Tyler J CurielWeiping Zou
Sep 11, 2004·Blood·Jose R Conejo-GarciaGeorge Coukos
Feb 16, 2005·Expert Opinion on Biological Therapy·Eduardo HuarteIgnacio Melero
Jun 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Fabian BenenciaGeorge Coukos
Jun 18, 2005·Human Gene Therapy·Fabian BenenciaGeorge Coukos
Oct 18, 2005·Cornea·Satoru YamagamiNobuyuki Ebihara
Nov 23, 2005·Oncology·Napoleone Ferrara
May 17, 2006·Clinical and Experimental Pharmacology & Physiology·P A MacaryX Dai
Jun 17, 2006·Current Opinion in Immunology·Roberto Bonasio, Ulrich H von Andrian
Oct 10, 2006·Cancer Metastasis Reviews·Michael R ShurinRobert L Ferris
Dec 16, 2006·Nature Reviews. Immunology·Ken Shortman, Shalin H Naik
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Paraic A KennyMina J Bissell
Aug 19, 2007·Trends in Immunology·Silvano SozzaniMarco Presta
Oct 5, 2007·Critical Reviews in Oncology/hematology·Paola AllavenaAlberto Mantovani
Jan 15, 2008·Cancer Immunology, Immunotherapy : CII·Takuya OsadaMichael A Morse
Apr 15, 2008·Investigative Ophthalmology & Visual Science·Kei NakaiRobert J D'Amato
Jul 18, 2008·Nature Reviews. Cancer·Craig MurdochClaire E Lewis
Sep 5, 2008·Cancer Research·Eduardo HuarteJose R Conejo-Garcia
Nov 26, 2008·Circulation Research·Israel GotsmanAndrew H Lichtman
Dec 17, 2008·Cancer Immunology, Immunotherapy : CII·Annabelle Grolleau-JuliusRaymond L Yung

❮ Previous
Next ❯

Citations

Apr 30, 2014·Frontiers in Oncology·Fabian BenenciaMawadda Alnaeeli
Oct 24, 2014·American Journal of Physiology. Cell Physiology·Venktesh S ShirureMonica M Burdick

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.