Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58.

The Journal of Biological Chemistry
M L Dustin

Abstract

The interaction of the T cell glycoprotein CD2 and its ligand CD58 is important for T cell interaction with antigen-presenting and target cells. The binding interaction is of low affinity and has a fast off-rate (>5 s-1) in solution. However, solution measurements may not accurately predict the behavior of molecules in an adhesive contact area. Interaction between T cells that express CD2 and glass-supported planar bilayers containing purified and fluorescently labeled CD58 leads to accumulation of CD58 (fluorescence) in the cell/bilayer contact area. CD58 molecules accumulated within the contact area in excess of the CD58 density in the bilayer outside the contact area can be considered as bound by cell surface CD2. Here, this phenomena and fluorescence photobleaching recovery were utilized to determine whether CD2-CD58 bonds are transient in contact areas. Fluorescent CD58 molecules accumulated in the T cell-bilayer interface were completely bleached. The bleached CD58 molecules accumulated in the contact area were rapidly replaced by fluorescent CD58 that diffused into the contact area from adjacent bilayer regions outside the contact area. Rapid recovery of the accumulated fluorescence directly demonstrates that the CD2-CD5...Continue Reading

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Citations

May 25, 2005·Annals of Biomedical Engineering·Jin-Yu ShaoMichael L Dustin
Apr 20, 2004·Cellular Signalling·Ortal ArielNurit Hollander
Dec 28, 1999·Journal of Biomechanics·F GuilakR A Brand
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Nov 1, 2001·Annual Review of Cell and Developmental Biology·M L DustinC Zhu
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May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elena V TibaldiEllis L Reinherz
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Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nadia AnikeevaYuri Sykulev
Apr 29, 2009·The Journal of Cell Biology·Yoshihisa KaizukaRonald D Vale
Jun 9, 2009·Journal of Structural Biology·Michael L Dustin
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Apr 9, 2008·Biophysical Journal·Timothy P TolentinoCheng Zhu
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Oct 4, 2007·The Journal of Biological Chemistry·Michael L DustinAdrian Whitty
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Jul 19, 2020·The FEBS Journal·Pablo F CéspedesErdinc Sezgin
Sep 4, 2015·Current Protocols in Cell Biology·Travis J CritesRajat Varma
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jun HuangCheng Zhu
Dec 16, 2006·ACS Chemical Biology·De-Min ZhuDavid E Golan
Jun 26, 2021·Frontiers in Immunology·Yalu ZhangQuan Liao
Sep 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kevin Y ChenSimon J Davis

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