Adhesive fiber stratification in uropathogenic Escherichia coli biofilms unveils oxygen-mediated control of type 1 pili

PLoS Pathogens
Kyle A FloydMaria Hadjifrangiskou

Abstract

Bacterial biofilms account for a significant number of hospital-acquired infections and complicate treatment options, because bacteria within biofilms are generally more tolerant to antibiotic treatment. This resilience is attributed to transient bacterial subpopulations that arise in response to variations in the microenvironment surrounding the biofilm. Here, we probed the spatial proteome of surface-associated single-species biofilms formed by uropathogenic Escherichia coli (UPEC), the major causative agent of community-acquired and catheter-associated urinary tract infections. We used matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) imaging mass spectrometry (IMS) to analyze the spatial proteome of intact biofilms in situ. MALDI-TOF IMS revealed protein species exhibiting distinct localizations within surface-associated UPEC biofilms, including two adhesive fibers critical for UPEC biofilm formation and virulence: type 1 pili (Fim) localized exclusively to the air-exposed region, while curli amyloid fibers localized to the air-liquid interface. Comparison of cells grown aerobically, fermentatively, or utilizing an alternative terminal electron acceptor showed that the phase-variable fim promoter swit...Continue Reading

References

Aug 1, 1984·Journal of Bacteriology·T K KorhonenJ Finne
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·D R Appling
Nov 5, 1999·Methods in Enzymology·G A O'TooleR Kolter
Jun 17, 2000·The EMBO Journal·J J MartinezS J Hultgren
Mar 5, 2003·Current Opinion in Microbiology·Kenneth G Campellone, John M Leong
May 20, 2003·The Journal of Biological Chemistry·Kirsty SalmonRobert P Gunsalus
Jul 5, 2003·Science·Gregory G AndersonScott J Hultgren
Dec 16, 2003·BMC Molecular Biology·Kenan C Murphy, Kenneth G Campellone
Jan 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sheryl S JusticeScott J Hultgren
Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·K Lewis
Oct 20, 2006·Current Opinion in Microbiology·Karen L Visick, Edward G Ruby
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jerome S PinknerFredrik Almqvist
Dec 5, 2006·Nature Reviews. Microbiology·Kim Lewis
May 18, 2007·Annual Review of Microbiology·Thomas Danhorn, Clay Fuqua
Sep 29, 2007·Nature Methods·Dale S CornettRichard M Caprioli
Dec 21, 2007·PLoS Medicine·David A RosenScott J Hultgren
Feb 21, 2008·Antimicrobial Agents and Chemotherapy·Rawya S Al-Dhaheri, L Julia Douglas
Feb 23, 2008·Rapid Communications in Mass Spectrometry : RCM·Martin StrohalmMilan Kodícek
May 13, 2008·Journal of the American Society for Mass Spectrometry·Erin H SeeleyRichard M Caprioli
Sep 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erin H Seeley, Richard M Caprioli
Oct 22, 2008·Current Opinion in Microbiology·Matthew R Parsek, Tim Tolker-Nielsen
Oct 13, 2009·Nature Reviews. Microbiology·Gabriel Waksman, Scott J Hultgren
Nov 4, 2009·Antimicrobial Agents and Chemotherapy·Gilly Regev-ShoshaniYossef Av-Gay
Nov 17, 2009·Nature Chemical Biology·Lynette CegelskiScott J Hultgren
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Swaine L ChenScott J Hultgren
Apr 15, 2010·Journal of Lipid Research·Veronika VidováJuha M Holopainen

❮ Previous
Next ❯

Citations

Jan 18, 2016·Journal of Microbiological Methods·Oleg V Podgorny, Vassili N Lazarev
Aug 11, 2015·Proteomics. Clinical Applications·Kyle A FloydMaria Hadjifrangiskou
Nov 6, 2015·Current Genetics·Surabhi Khandige, Jakob Møller-Jensen
Apr 19, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Johan ZakrissonMagnus Andersson
Oct 25, 2016·Environmental Microbiology·Fanny LarsonneurChristophe Beloin
Mar 24, 2017·MicrobiologyOpen·Courtney M AbanaMaria Hadjifrangiskou
Jan 28, 2018·The Journal of Membrane Biology·Bradley D SteinerMaria Hadjifrangiskou
Oct 5, 2017·International Journal of Molecular Sciences·Allison R EberlyMaria Hadjifrangiskou
Feb 24, 2016·Pathogens·Sudha MoorthyEric A Klein
Nov 1, 2016·Infection and Immunity·Carrie L ShafferMaria Hadjifrangiskou
Mar 5, 2016·Microbiology and Molecular Biology Reviews : MMBR·Amelia E BarberMatthew A Mulvey
Mar 16, 2016·Journal of Bacteriology·Karen L VisickJean-Marc Ghigo
Nov 1, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Natasha Naidoo, Manormoney Pillay
Sep 12, 2019·Frontiers in Microbiology·Karishma Bisht, Catherine Ann Wakeman
May 5, 2017·Scientific Reports·Stefan BehrKirsten Jung
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
May 26, 2017·Frontiers in Cellular and Infection Microbiology·Erin J BrelandMaria Hadjifrangiskou
Feb 21, 2019·Journal of Bacteriology·Clay FuquaKaren L Visick
Jun 6, 2020·Nature Communications·John R BrannonMaria Hadjifrangiskou
Jan 30, 2021·Nature Reviews. Gastroenterology & Hepatology·Jean-Paul MottaNathalie Vergnolle
Mar 18, 2021·NPJ Biofilms and Microbiomes·Karishma BishtCatherine A Wakeman
Apr 18, 2021·NPJ Biofilms and Microbiomes·Connor J BeeboutMaria Hadjifrangiskou
Mar 15, 2018·EcoSal Plus·Glenn T Werneburg, David G Thanassi
Jul 18, 2015·Microbiology Spectrum·Ian C Blomfield
Jul 3, 2021·Viruses·Stephen T AbedonMatthew B Sullivan

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
transmission electron microscopy
Structured
Illumination Microscopy
PCR

Software Mentioned

Fiji Image J
mMass
GraphPad
FlexImaging
Adobe Photoshop
Photoshop
SoftWorx
SoftWorx for OMX
GraphPad Prism
Volume Viewer

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.