Abstract
This research aims at evaluating the expression of some adhesive proteins on endothelial cell surface with polyethylene terephthalate coated with pyrolytic carbon (PET + PC). Twenty-two different cultures of human umbilical vein endothelial cells (HUVECs) were put in contact with PET + PC. Both HUVECs grown without the biomaterial and HUVECs incubated with endotoxin were used as control. After 24 h, platelet endothelial cell adhesion molecule-1 (PECAM-1), endothelial leukocyte adhesion molecule-1 (ELAM-1), intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were evaluated on the cells by monoclonal antibodies and flow cytometry. In agreement with the literature, after 24 h the culture incubated with endotoxin determined a significant increase in the percentage of positive cells for ELAM-1, a significant increase in fluorescence intensity of ICAM-1, and a significant increase in the percentage of positive cells and fluorescence intensity for VCAM-1. After 24 h of culture with PET + PC, no significant variations in the antigens examined were observed. This demonstrates that such material does not activate in vitro the proteins involved in the adhesion between leucocytes and endothelium or in ...Continue Reading
References
Jan 1, 1979·Biomaterials, Medical Devices, and Artificial Organs·A PizzoferratoS Freeman
Sep 1, 1991·The Journal of Cell Biology·S M AlbeldaP J Newman
Aug 2, 1990·Nature·T A Springer
Jul 13, 1990·Cell·L Osborn
Apr 1, 1990·Immunological Reviews·T M Carlos, J M Harlan
Apr 1, 1989·Biomaterials·K Kottke-MarchantR E Marchant
Sep 1, 1989·Biomaterials·D L LeakeA Pizzoferrato
Jun 1, 1987·Journal of Biomedical Materials Research·P B van WachemW G van Aken
Mar 1, 1985·Biomaterials·R BarbucciF Tempesti
Nov 1, 1987·Journal of Biomedical Materials Research·P B van WachemW G van Aken
Nov 1, 1986·Journal of Biomedical Materials Research·S D CookA D Haubold
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeC R Minick
Jan 1, 1993·Plastic and Reconstructive Surgery·G BocchiottiE Robotti
Sep 1, 1995·Biomaterials·E CenniA Pizzoferrato
Mar 1, 1997·Biomaterials·E CenniA Pizzoferrato
Citations
May 16, 2002·Biomaterials·F R PuJ A Hunt
Mar 1, 1997·Biomaterials·E CenniA Pizzoferrato
Jul 22, 1998·Progress in Neurobiology·P Heiduschka, S Thanos
Aug 31, 2002·Biomolecular Engineering·A AuditoreG Marletta
Nov 2, 2005·Tissue Engineering·Wei HeSeeram Ramakrishna
Oct 6, 2001·Journal of Biomaterials Science. Polymer Edition·N SheikhS Amanpour
Dec 22, 1999·Journal of Biomaterials Science. Polymer Edition·E CenniL Montanaro
Jul 16, 2014·TheScientificWorldJournal·Ilaria ArmentanoLivia Visai
Jul 20, 2005·Archives of Physiology and Biochemistry·Y J T van der ZijppJ Feijen
Feb 24, 2007·Biomaterials·Alison P McGuigan, Michael V Sefton
May 15, 2003·Journal of Biomedical Materials Research. Part a·Karla LehleDietrich E Birnbaum
Mar 23, 2005·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Mila Della BarberaMarialuisa Valente
Nov 25, 2003·Journal of Interventional Cardiology·Antonio L BartorelliPaolo Ravagnani
Mar 14, 2003·Journal of Biomedical Materials Research. Part a·Ype J T van der ZijppJan Feijen
Apr 12, 2014·BioMed Research International·Duk-Yeon KimSukyoung Kim
Jan 13, 2015·Materials Science & Engineering. C, Materials for Biological Applications·M M MachadoM A F Corat
Apr 23, 2005·American Heart Journal·Young-Hak KimSeung-Jung Park
May 17, 2006·Acta Biomaterialia·Rui ZhuoErwin A Vogler
Oct 20, 2012·The International Journal of Artificial Organs·Carla Renata ArciolaLucio Montanaro
May 24, 2001·Journal of Biomedical Materials Research·E ImbertJ Feijen
Jul 11, 2018·Materials·Taemin KimKi Jun Yu
Jun 5, 1998·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·C J KirkpatrickM Otto