Adhesiveness of mononuclear cells in hypercholesterolemic humans is normalized by dietary L-arginine

Arteriosclerosis, Thrombosis, and Vascular Biology
G TheilmeierJ P Cooke

Abstract

Hypercholesterolemia reduces vascular nitric oxide (NO) activity. This dysfunction may promote endothelial monocyte interaction, as NO is a potent inhibitor of cell adhesion. We have previously shown that in hypercholesterolemic (HC) rabbits, chronic oral supplementation of L-arginine (Arg) restores NO activity and inhibits monocyte-endothelial cell interaction, in association with a reduction in atherogenesis. We hypothesized that enhancement of endothelial NO activity in HC humans would reduce monocyte adhesiveness. We used a functional binding assay to assess the adhesiveness of human mononuclear cells (MNCs) ex vivo to determine the effects of hypercholesterolemia and L-arginine administration. MNCs from HC subjects adhered in greater numbers (50% more cells per high-power field; P < .0001) than cells derived from normocholesterolemic (NC) subjects. To determine whether enhancement of endogenous NO activity could inhibit mononuclear cell adhesiveness, in a double-blinded placebo-controlled study, oral arginine HCl (8.4 g/d) was administered to HC subjects. Over a course of 2 weeks, this treatment abolished the increased adhesiveness of HC MNCs (160 +/- 11% versus 104 +/- 5%; before and after 2 weeks of Arg treatment; result...Continue Reading

References

Jun 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K Takahasi, Y Sawasaki
Oct 1, 1992·The Journal of Clinical Investigation·M A CreagerJ P Cooke
Sep 1, 1992·The Journal of Clinical Investigation·J P CookeM E Billingham
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P KubesD N Granger
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S D CushingA M Fogelman
Dec 1, 1990·The Journal of Clinical Investigation·R L MinorD G Harrison
Jan 1, 1986·The Journal of Nutrition·W J Visek
Feb 20, 1986·The New England Journal of Medicine·R Ross
Oct 1, 1995·Journal of the American College of Cardiology·M R AdamsD S Celermajer
Apr 18, 1994·Annals of the New York Academy of Sciences·A WennmalmF Waagstein
Jun 1, 1993·The Journal of Clinical Investigation·Y OharaD G Harrison
Apr 1, 1993·The Journal of Clinical Investigation·J F KeaneyJ Loscalzo
Dec 1, 1996·British Journal of Pharmacology·R J MacAllisterP Vallance

❮ Previous
Next ❯

Citations

Feb 24, 2000·The Journal of Pathology·H Li, U Förstermann
Dec 11, 2002·The Journal of Pathology·Ester W J A AlbrechtHarry van Goor
Apr 5, 2001·Current Atherosclerosis Reports·J P Cooke, R K Oka
Apr 12, 2002·Atherosclerosis·Robert B PreliDavid M Herrington
Dec 29, 2000·Journal of the American College of Cardiology·R H BögerJ P Cooke
Sep 1, 1999·European Journal of Clinical Investigation·N de BontA F Stalenhoef
Mar 10, 2000·Journal of Cardiovascular Pharmacology·C ArtiguesC Thuillez
Jan 22, 2002·Current Opinion in Lipidology·D M Colquhoun
Aug 3, 1999·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·M PfeiferB Zizek
Jul 25, 2006·International Journal of Cardiology·Gerasimos SiasosChristodoulos Stefanadis
Jun 21, 2001·The Annals of Pharmacotherapy·J W ChengS N Balwin
Nov 26, 1999·Annals of Medicine·L NittynenH Vapaatalo
Mar 29, 2001·The American Journal of Clinical Nutrition·A A Brown, F B Hu
Dec 29, 2006·Journal of Atherosclerosis and Thrombosis·Taro Kuwai, Junichi Hayashi
Jul 1, 2012·Indian Journal of Clinical Biochemistry : IJCB·Pratima TripathiShivani Pandey
Jan 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Rayomand S KhambataAmrita Ahluwalia
Oct 7, 2004·The Journal of Nutrition·Heather L Gornik, Mark A Creager
Sep 8, 2004·The Journal of Nutritional Biochemistry·Rhobert W EvansLewis H Kuller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.