Adipocyte aquaporin 7 (AQP7) expression in lean children and children with obesity. Possible involvement in molecular mechanisms of childhood obesity

Journal of Pediatric Endocrinology & Metabolism : JPEM
Eleni OikonomouBessie E Spiliotis

Abstract

Background Aquaporin 7 (AQP7), a water/glycerol transporting protein, regulates adipocyte glycerol efflux and influences lipid and glucose homeostasis. Altered AQP7 expression in adults leads to impaired glycerol dynamics, adipocyte hypertrophy and it predisposes them to obesity and diabetes. To assess its possible involvement in childhood obesity, this study investigated the expression of adipocyte AQP7 in cultured adipocytes of children. Methods Primary in vitro differentiated adipocyte cultures were developed from surgical biopsies of subcutaneous abdominal adipose tissue from 61 (46 prepubertal, 15 pubertal) lean children (body mass index [BMI] <85%) and 41 (22 prepubertal, 19 pubertal) children with obesity (BMI >95%). AQP7 expression was studied by reverse transcription polymerase chain reaction and Western immunoblotting and insulin by enzyme-linked immunosorbent assay. Results AQP7 messenger RNA (mRNA) was increased in the younger obese prepubertal (YOP) children but decreased in the obese adolescents (OA) (p=0.014) who also had increased insulin and homeostatic model assessment - insulin resistance (HOMA-IR). Lean pubertal (LP) children and YOP had increased 41 kDa AQP7 protein expression (p=0.001 and p=0.005, respecti...Continue Reading

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Citations

Oct 8, 2019·Hormones : International Journal of Endocrinology and Metabolism·Roza MourelatouBessie E Spiliotis
Mar 9, 2020·Hormones : International Journal of Endocrinology and Metabolism·Eleni OikonomouBessie E Spiliotis
Jan 23, 2020·International Journal of Molecular Sciences·Inês Vieira da SilvaGraça Soveral
Mar 3, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yawei ShenXiaowu Chen
Mar 11, 2021·Biochimie·Giuseppe Calamita, Christine Delporte
Jun 6, 2021·Redox Biology·Mauro GalliPiotr Zabielski

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