Adipogenic differentiation of human adipose tissue-derived stem cells obtained from cryopreserved adipose aspirates

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]
Jung Eun LeeMihyung Kim

Abstract

Although frozen adipose tissue is frequently used for soft tissue augmentation, the viability of frozen fat remains a controversy. The cryopreservation of adipose tissue is important for the future use of adipose-derived stem cells (ASCs) and adipocytes. To determine whether optimal cryopreservation techniques with regard to the addition of cryopreservative agents and preservation temperature is essential for the long-term storage of adipose tissue and whether ASCs from cryopreserved adipose aspirates are reliable for use in adipogenic differentiation. Adipose tissue was frozen directly or with cryoprotectant at -20 degrees C or -80 degrees C for 1 year. The viability of adipose aspirates and the differentiation of ASCs isolated from adipose tissue were evaluated. The viability of adipose aspirates frozen with dimethyl sulfoxide at -80 degrees C was approximately 87% after 2 months of storage. Moreover, ASCs from adipose tissue stored with cryoprotectant survived successfully for 1 year and differentiated into adipocytes, although ASCs were not detected in the directly frozen adipose tissue. Adipose tissue cryopreserved with cryoprotectant and stored at optimal temperature might prove to be a reliable source of human ASCs and a...Continue Reading

References

Sep 2, 1998·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·J E FultonT Barnes
Jan 3, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·P F Fournier
Jan 3, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·G Sattler, B Sommer
Jan 3, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·S Eremia, N Newman
May 10, 2003·Facial Plastic Surgery : FPS·Suhail K Kanchwala, Louis P Bucky
Sep 28, 2005·Annals of Plastic Surgery·Timm P WolterN Pallua
Oct 21, 2005·The Keio Journal of Medicine·Brian M StremMarc H Hedrick
Jan 19, 2006·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·David K MoscatelloNaomi Lawrence
May 3, 2006·Plastic and Reconstructive Surgery·Lee L Q PuHenry C Vasconez
May 13, 2006·Biochemical and Biophysical Research Communications·Seung-Woo ChoByung-Soo Kim
Aug 29, 2006·Plastic and Reconstructive Surgery·Sydney R Coleman
Aug 19, 2007·Plastic and Reconstructive Surgery·Lee L Q Pu

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Citations

Apr 30, 2011·Stem Cells and Development·Michelle A ScottAaron W James
Feb 16, 2012·Lasers in Surgery and Medicine·Samantha Y ShekHenry H Chan
Aug 24, 2013·Aesthetic Surgery Journal·Wei Z WangWilliam A Zamboni
Apr 24, 2014·Aesthetic Surgery Journal·O Onur Erol

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