Adiponectin and leptin are secreted through distinct trafficking pathways in adipocytes

Biochimica Et Biophysica Acta
Linglin XieSilvia Mora

Abstract

Adiponectin and leptin are two adipokines secreted by white adipose tissue that regulate insulin sensitivity. Previously we reported that adiponectin but not leptin release depends on GGA-coated vesicle formation, suggesting that leptin and adiponectin may follow different secretory routes. Here we have examined the intracellular trafficking pathways that lead to the secretion of these two hormones. While adiponectin and leptin displayed distinct localization in the steady-state, treatment of adipocytes with brefeldin A inhibited both adiponectin and leptin secretion to a similar level, indicating a common requirement for class III ADP-ribosylating factors and an intact Golgi apparatus. Adiponectin secretion was significantly reduced by endosomal inactivation in both 3T3L1 and rat isolated adipocytes, whereas this treatment had no effect on leptin secretion. Importantly, endosomal inactivation completely abolished the insulin stimulatory effect on adiponectin release in rat adipocytes. Confocal microscopy studies revealed colocalization of adiponectin with endogenous rab11 a marker for the recycling endosome, and with expressed rab5-GFP mutant (rab5Q75L) a marker for the early endosome compartment. Colocalization of adiponectin...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Oct 30, 1989·Biochimica Et Biophysica Acta·K KitagawaL I Tanner
Nov 10, 1995·The Journal of Biological Chemistry·P E SchererH F Lodish
Feb 1, 1996·The New England Journal of Medicine·R V ConsidineT L Bauer
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·N MorinagaM Vaughan
May 1, 1997·The Journal of Clinical Endocrinology and Metabolism·S Papaspyrou-RaoS K Fried
Dec 17, 1997·The Journal of Clinical Endocrinology and Metabolism·W T DonahooR H Eckel
Feb 7, 1998·The Journal of Biological Chemistry·T Iglesias, E Rozengurt
Apr 29, 1999·Cell·P Chardin, F McCormick
Jul 6, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·C CouillardJ P Després
Mar 10, 2001·Recent Progress in Hormone Research·R L BradleyB Cheatham
Jul 14, 2001·The Journal of Biological Chemistry·C RohK V Kandror
Aug 2, 2001·Nature Medicine·A H BergP E Scherer
Nov 9, 2001·Biochemical and Biophysical Research Communications·C M HalleuxS M Brichard
Dec 19, 2001·The Journal of Clinical Investigation·T P CombsL Rossetti
Jan 18, 2002·Nature·Yasuhiko MinokoshiBarbara B Kahn
Dec 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Hiroyuki MotoshimaBarry J Goldstein
Feb 13, 2003·Science's STKE : Signal Transduction Knowledge Environment·Paul Trayhurn
May 24, 2003·Current Diabetes Reports·Utpal B Pajvani, Philipp E Scherer
Dec 6, 2003·Pediatric Diabetes·O Moran, M Phillip
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Juan S Bonifacino
Mar 19, 2004·Diabetes & Metabolism·M Guerre-Millo
Apr 3, 2004·Current Drug Targets·Susanne Klaus
Sep 24, 2004·Journal of Pediatric Endocrinology & Metabolism : JPEM·Frank B DiamondDuane Eichler
Mar 3, 2005·American Journal of Physiology. Endocrinology and Metabolism·Philippe G CammisottoLudwik J Bukowiecki
Aug 20, 2005·Trends in Cell Biology·Yashoda Ghanekar, Martin Lowe
Jan 13, 2006·The Journal of Biological Chemistry·Linglin XieSilvia Mora
Mar 7, 2006·Biochemical and Biophysical Research Communications·Mairi ClarkeGwyn W Gould
Nov 24, 2006·American Journal of Physiology. Endocrinology and Metabolism·Mi-Jeong LeeSusan K Fried

❮ Previous
Next ❯

Citations

Dec 20, 2013·International Journal of Obesity : Journal of the International Association for the Study of Obesity·I SchlottmannV Lamounier-Zepter
Jan 22, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S KralischM Fasshauer
Sep 3, 2009·Molecular and Cellular Endocrinology·Sandra GalicGregory R Steinberg
Apr 21, 2009·Regulatory Peptides·Ninna Bo NielsenBente Stallknecht
May 27, 2008·Experimental Cell Research·Anja ZeigererJeffrey M Friedman
Feb 13, 2013·American Journal of Medical Genetics. Part a·Carla Sustek D'AngeloCelia Priszkulnik Koiffmann
Feb 6, 2014·Acta Physiologica·T RomachoJ Eckel
Apr 23, 2015·Biochimica Et Biophysica Acta·Vanessa DeClercqRoger S McLeod
Nov 1, 2011·Progress in Neurobiology·Xueyi Li, Marian DiFiglia
Jun 7, 2016·Biochemical and Biophysical Research Communications·Ju CuiTie-Mei Zhang
Nov 9, 2017·The Journal of Endocrinology·Gulizar Issa Ameen, Silvia Mora
Sep 11, 2008·American Journal of Physiology. Endocrinology and Metabolism·Giuseppe MurdoloPer-Anders Jansson
Jul 25, 2019·International Journal of Molecular Sciences·Thomas GrewalChrista Buechler
Feb 19, 2010·Clinical and Vaccine Immunology : CVI·Linglin XieStephen K Chapes
Sep 16, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Mark C RentonKirsten F Howlett
Sep 27, 2018·Molecular and Cellular Biochemistry·Chian Ju JongMorris Karmazyn
Jun 1, 2013·Hepatology International·Carmine Finelli, Giovanni Tarantino
Jul 24, 2010·Journal of Endocrinological Investigation·S KouidhiA Benammar Elgaaied
Apr 1, 2017·Chinese Medicine·Shinjiro KobayashiMasaaki Nomura
Apr 1, 2021·Hormones : International Journal of Endocrinology and Metabolism·Zahra GaeiniZahra Bahadoran
Oct 16, 2020·Metabolism: Clinical and Experimental·Anouar Hafiane, Stella S Daskalopoulou
Aug 13, 2021·Journal of Cell Science·Benjamin S RobertsSaskia B Neher
Aug 28, 2021·International Journal of Molecular Sciences·Marina Ruxandra OteleaAgripina Rascu
May 13, 2009·Current Opinion in Lipidology
Oct 22, 2009·Science Signaling·Pearl V Ryder, Victor Faundez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.