Adiponectin Is Involved in Connective Tissue Growth Factor-Induced Proliferation, Migration and Overproduction of the Extracellular Matrix in Keloid Fibroblasts

International Journal of Molecular Sciences
Limin LuoChundi He

Abstract

Adiponectin, an adipocyte-derived hormone, exerts pleiotropic biological effects on metabolism, inflammation, vascular homeostasis, apoptosis and immunity. Recently, adiponectin has been suggested to attenuate the progression of human dermal fibrosis. Connective tissue growth factor (CTGF) is induced in keloids and is thought to be participated in the formation of keloid fibrosis. However, the roles played by adiponectin in keloids remain unclear. In this study, we explored the effects of adiponectin on CTGF-induced cell proliferation, migration and the deposition of extracellular matrix (ECM) and their associated intracellular signalling pathways in keloid fibroblasts (KFs). We also explored possible mechanisms of keloid pathogenesis. Primary fibroblast cultures were established from foreskin biopsies and skin biopsies from patients with keloids. The expression of adiponectin and adiponectin receptors (adipoRs) was evaluated by reverse transcription-PCR (RT-PCR), quantitative real-time RT-PCR, immunofluorescence staining, and immunohistochemical analysis. Next, KFs and normal dermal fibroblasts (NFs) were treated with CTGF in the presence or absence of adiponectin. A cell counting kit-8 (CCK-8) and the Transwell assay were use...Continue Reading

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Citations

Apr 25, 2020·International Journal of Molecular Sciences·Claudia C DarmawanJe-Ho Mun
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Nov 13, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ilja L KruglikovPhilipp E Scherer

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Methods Mentioned

BETA
gene knockdown
biopsies
circumcision
reverse transcription PCR
reverse transcription-PCR
PCR
protein assay
electrophoresis
fluorescence microscopy

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