Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis

Nature Metabolism
Camille ArcinasLei Sun

Abstract

Non-coding RNAs are emerging as novel regulators in adipocyte differentiation and function. Circular RNAs (circRNAs) are a new class of non-coding transcripts generated across all eukaryotic tissues, but their function in adipose biology remains unknown. Here we perform deep sequencing of visceral and subcutaneous fat to discover thousands of adipose circRNAs, many of which are species conserved, tissue specific and dynamically regulated during adipogenesis and obesity. We identify circTshz2-1 and circArhgap5-2 as indispensable regulators of adipogenesis in vitro. To characterize the function of circRNAs in vivo, we deliver adenoviral shRNA targeting circArhgap5-2 into mouse inguinal tissue and show that the expression of this circRNA is essential in maintaining the global adipocyte transcriptional programme involved in lipid biosynthesis and metabolism. We also demonstrate that the pro-adipogenic function of circArhgap5-2 is conserved in human adipocytes. Our results provide important evidence that circRNAs serve as important regulators in adipocyte differentiation and metabolism.

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Citations

Oct 19, 2020·Journal of Hematology & Oncology·Xiangxiang ZhouXin Wang
Dec 22, 2020·European Journal of Pharmacology·Soudeh Ghafouri-Fard, Mohammad Taheri
Jan 15, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Federica ReyStephana Carelli
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Xuemei ShenHong Chen
Dec 5, 2020·Journal of Cellular Physiology·Guangli YuYuncheng Lv
Jun 5, 2021·Archives of Physiology and Biochemistry·Sadra Samavarchi TehraniReza Meshkani
Jul 25, 2021·International Journal of Molecular Sciences·Caterina FormichiFrancesco Dotta
Jul 22, 2021·Journal of Molecular Cell Biology·Wei GaoQin Yang
Nov 9, 2021·Frontiers in Genetics·Pengpeng ZhangYongjie Xu

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Datasets Mentioned

BETA
GSE22004

Methods Mentioned

BETA
RNA-seq
PCR
GTPase
immunoprecipitation
pull down
electrophoresis
density gradient centrifugation

Software Mentioned

Leica Application Suite X
Cufflinks2
CIRI2
– MEM )
BWA
ImageJ
GSEA
GENCODE
edgeR
Burrows Aligner (

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