Adipose-derived cells improve left ventricular diastolic function and increase microvascular perfusion in advanced age

PloS One
Natia Q KelmAmanda J LeBlanc

Abstract

An early manifestation of coronary artery disease in advanced age is the development of microvascular dysfunction leading to deficits in diastolic function. Our lab has previously shown that epicardial treatment with adipose-derived stromal vascular fraction (SVF) preserves microvascular function following coronary ischemia in a young rodent model. Follow-up studies showed intravenous (i.v.) delivery of SVF allows the cells to migrate to the walls of small vessels and reset vasomotor tone. Therefore we tested the hypothesis that the i.v. cell injection of SVF would reverse the coronary microvascular dysfunction associated with aging in a rodent model. Fischer 344 rats were divided into 4 groups: young control (YC), old control (OC), old + rat aortic endothelial cells (O+EC) and old + GFP+ SVF cells (O+SVF). After four weeks, cardiac function and coronary flow reserve (CFR) were measured via echocardiography, and hearts were explanted either for histology or isolation of coronary arterioles for vessel reactivity studies. In a subgroup of animals, microspheres were injected during resting and dobutamine-stimulated conditions to measure coronary blood flow. GFP+ SVF cells engrafted and persisted in the myocardium and coronary vasc...Continue Reading

References

May 16, 2002·Clinical and Experimental Pharmacology & Physiology·Hanzhong LiuRichard Laverty
Apr 27, 2005·Circulation·Michael HofmannHelmut Drexler
Jun 23, 2009·Trends in Pharmacological Sciences·Michael A HillIsmail Laher
Oct 9, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Amanda J LeBlancJudy M Muller-Delp
Jul 10, 2010·Antioxidants & Redox Signaling·Kathryn H YuillKim A Dora
Mar 29, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Lori S KangJudy M Muller-Delp
Dec 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Amanda J LeblancStuart K Williams
May 29, 2012·Plastic and Reconstructive Surgery·Phanette GirRod J Rohrich
Oct 17, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sandra De BarrosValérie Planat-Bénard
Dec 4, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Amanda J LeblancJudy M Muller-Delp
Jan 4, 2013·Stem Cells and Development·Sarah T HsiaoRodney J Dilley
Oct 10, 2013·Stem Cells Translational Medicine·Amanda J LeblancStuart K Williams
Jan 15, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Zhuo ZhaoLeanne Groban
May 23, 2014·Current Vascular Pharmacology·Ana M BrionesMercedes Salaices
Jul 10, 2014·PloS One·Nils Thomas SongstadGanesh Acharya
Feb 14, 2015·JACC. Cardiovascular Imaging·Mark A MarinescuJamieson M Bourque
Mar 12, 2016·Journal of the American College of Cardiology·Joo Myung LeeBon-Kwon Koo
May 21, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Chris NevittAmanda LeBlanc
Oct 18, 2016·Current Opinion in Cardiology·Viviany R Taqueti, Marcelo F Di Carli
Jan 3, 2018·European Heart Journal·Viviany R TaquetiMarcelo F Di Carli
Feb 10, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Kathryn DryerJohn E A Blair

❮ Previous
Next ❯

Citations

Sep 24, 2019·Expert Opinion on Biological Therapy·Isabel AndiaNatalia Burgos-Alonso
Jan 23, 2020·Journal of Clinical Medicine·Ruxandra I SavaKeith L March
May 12, 2020·Immunology and Cell Biology·Katlin B StiversJames B Hoying
May 13, 2020·FASEB BioAdvances·Natia Q KelmAmanda J LeBlanc
Jul 30, 2020·PloS One·Natia Q KelmSham S Kakar

❮ Previous
Next ❯

Methods Mentioned

BETA
coronary artery bypass
dissection
fluorescence microscopy

Software Mentioned

Nikon NIS Elements AR
ImageJ
Mode
Lab Chart EKG Analysis Module
ImageJ Plugins
SPSS
Olympus cellSens
VEVO ® LAB

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.