Adipose-derived mesenchymal stem cells release microvesicles with procoagulant activity

The International Journal of Biochemistry & Cell Biology
Tomas FiedlerKirsten Peters

Abstract

Extracellular vesicles are produced by a number of different cell types, among them mesenchymal stromal/stem cells (MSC) of different sources. It has been shown that extracellular vesicles of MSC exert similar therapeutic effects as the cells themselves. Here, we isolated and characterized extracellular vesicles produced by adipose-derived MSC (adMSC) in vitro upon stimulation with the proinflammatory substances lipopolysaccharide (LPS) and tumor necrosis factor (TNF). We found that the number of vesicles produced by adMSC does not change upon stimulation of the cells with LPS and TNF. Furthermore, adMSC-derived extracellular vesicles exert procoagulant activity independent of previous stimulation with LPS or TNF. We found evidence that the vesicles induce coagulation via both the intrinsic and the extrinsic pathway of coagulation.

Citations

Jul 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhuo LiangYonggang Lv
Apr 26, 2019·Cell Transplantation·Aleksandra Musiał-WysockaMarcin Majka
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Marie MaumusDanièle Noël
Sep 10, 2020·American Journal of Respiratory and Critical Care Medicine·Jonathan E MillarUNKNOWN all authors of ‘Combined Mesenchymal Stromal Cell Therapy and Extracorporeal Membrane Oxygenation in Acute Respiratory Distr
Aug 29, 2021·Pharmaceuticals·Deimantė NarauskaitėAistė Jekabsone

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