Adipose-derived mesenchymal stromal cells suppress osteoclastogenesis and bone erosion in collagen-induced arthritis.

Scandinavian Journal of Immunology
Qing ChangJun-Zheng Hu

Abstract

Osteoclasts are responsible for bone destruction in rheumatoid arthritis (RA), and adipose-derived mesenchymal stromal cells (ADSCs) can inhibit experimental collagen-induced arthritis model. This study aims to determine whether ADSCs also suppresses osteoclastogenesis and bone erosion in collagen-induced arthritis (CIA). Osteoclasts were induced from bone marrow-derived CD11b+ cells with receptor activator of nuclear factor-κ B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) stimulation and assessed with tartrate-resistant acid phosphatase (TRAP) staining. For human cells, osteoclasts were produced from human CD14+ cells. ADSCs were generated and added to cultures with different ratios with CD11b+ cells. Transwell and antibody blockade experiments were performed to define the mechanism of action. NF-κB and RANKL expression were determined by Western blotting and RT-qPCR. About 2 × 106 ADSCs or fibroblast cells were adoptively transferred to DBA1/J mice on day 14 after immunization with type II collagen/complete Freund's adjuvant (CII/CFA) while the onset and severity of the CIA were monitored. Adipose-derived mesenchymal stromal cells but not fibroblast cells completely suppressed osteoclastogenesis in vitro fo...Continue Reading

References

Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Mar 16, 2012·Immunologic Research·Doreen HaaseOlaf Rotzschke
Jan 17, 2014·Annals of the Rheumatic Diseases·Katerina ChatzidionysiouUNKNOWN ARTIS group
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Jun 27, 2014·BioMed Research International·Anne Halenius, Hartmut Hengel

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Citations

Jan 26, 2021·Scandinavian Journal of Immunology
May 6, 2021·Biomolecules·Mariachiara ZuccariniPatrizia Di Iorio

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