Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes

Osteoarthritis and Cartilage
LiBo JiangJian Zhang

Abstract

Adipose-derived stem cells (ADSCs) have been demonstrated to have an anti-apoptosis effect on chondrocytes; However, their effect on autophagic activation remains unclear. We sought to explore whether ADSCs can activate autophagy and inhibit IL-1β- and lipopolysaccharide (LPS)-induced catabolism in chondrocytes. ADSCs and chondrocytes were collected from SD rats. The biologic characteristics of ADSCs were analyzed by flow cytometric analysis, Oil red O and Alizarin Red staining. Autophagic level and autophagic flux were revealed by Western blotting for LC3-II and SQSTM1/P62, MDC (monodansylcadaverine) staining and mRFP-GFP-LC3 analysis. The mTOR pathway was investigated by Western blotting for p-mTOR. The mRNA level of matrix metalloproteinases (MMPs) and thrombospondin motifs (ADAMTSs) was detected by real-time PCR. The typical surface markers and differentiation potentials of ADSCs were proved. ADSCs enhanced the expression of LC3-II/LC3-I and reduced SQSTM1 levels in IL-1β-induced chondrocytes after 24 and 48 h co-culturing and in LPS-induced chondrocytes after 48 h co-culturing respectively. mRFP-GFP-LC3 analysis suggested that autophagosomes and autolysosomes were formed earlier in IL-1β-treated chondrocytes than in LPS-tr...Continue Reading

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Citations

Jan 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yaping YeTao Xu
Jul 11, 2018·Frontiers in Physiology·Claire VinatierBeatriz Caramés
Aug 25, 2018·BioMed Research International·Fan YangChangqing Zhang

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