Abstract
To evaluate the ability of human adipose-derived stem cells (h-ASCs) to survive and differentiate in corneal stroma. Our experiment consisted of 2 phases. First, we cultured h-ASCs in different types of hyaluronic acid (HA)-derived synthetic extracellular matrixes (sECMs) to determine the capability of proliferation and survival of the cells in hydrogels. Second, h-ASCs were grown in plastic flasks, labeled with an intracytoplasmic membrane fluorescent molecule, transferred onto different types of sECMs or the native HA product, and then inserted into the corneal stroma of the rabbits. After 10 weeks, we assessed the viability of the stem cells and the expression of cornea-specific proteins. The in vitro study showed that the HyStem-HP hydrogel had the highest yield of cells (1.1 × 10(6)/mL) compared with other types of HA-derived sECMs culture media, and the cells grown in the HyStem-HP hydrogel appeared more elongated and fibroblastlike. The in vivo study demonstrated that the labeled h-ASCs could be identified in the stroma with any type of sECM. The HA-derived sECMs, particularly the HyStem-HP hydrogel, showed better survival and cell morphologic features compared with pure HA. Immunostaining of keratocan, aldehyde dehydrog...Continue Reading
Citations
Jul 26, 2014·Journal of Biological Engineering·Timothy N SnyderDaewon Park
Nov 6, 2014·Advances in Wound Care·Barbara WirostkoGlenn D Prestwich
Jan 25, 2013·International Journal of Nanomedicine·Eunna ChungLaura J Suggs
Jan 20, 2016·Eye and Vision·Francisco Arnalich-MontielJorge L Alió
Apr 16, 2015·Journal of Clinical Periodontology·Karim M Fawzy El-SayedChristof E Dörfer
Feb 13, 2013·Carbohydrate Polymers·Maurice N Collins, Colin Birkinshaw
Oct 7, 2016·Advanced Healthcare Materials·Helena Knopf-MarquesPhilippe Lavalle
May 10, 2017·Cornea·Jorge L Alió Del BarrioJorge L Alió
Sep 7, 2016·Journal of Tissue Engineering and Regenerative Medicine·Pramod KumarDimitrios I Zeugolis
Oct 10, 2013·Journal of Neuroscience Research·Alan D WidgerowGregory R D Evans
Jun 10, 2014·Journal of Biomedical Materials Research. Part a·Jorge L Alió Del BarrioMaria P De Miguel
Jun 6, 2013·Biomedical Materials·Veronika HruschkaSusanne Wolbank
Mar 31, 2016·Advanced Materials·Pramod KumarDimitrios I Zeugolis
Jan 1, 2014·Microsurgery·Alan D WidgerowGregory R D Evans
Aug 24, 2017·Scientific Reports·Molly R Kelly-GossShayn M Peirce
Nov 10, 2018·Eye and Vision·Jorge L Alió Del Barrio, Jorge L Alió
Dec 19, 2018·Histochemistry and Cell Biology·Julia FuchsDagmar Brislinger
Nov 18, 2014·Stem Cells·Damien G HarkinBrendan G Cronin
Feb 28, 2018·Polymers·Janine GriesserAndreas Bernkop-Schnürch
Apr 9, 2020·International Journal of Molecular Sciences·Jana PryjmakováJakub Siegel
Nov 12, 2020·Biomaterials Science·Zoe HancoxFarshid Sefat
Nov 13, 2020·Asia-Pacific Journal of Ophthalmology·M El ZarifJorge L Alió
Feb 5, 2021·Experimental Eye Research·Hossein AghamollaeiKhosrow Jadidi
Feb 25, 2021·International Ophthalmology·Adriano FasoloGiorgio Marchini
Mar 13, 2021·Frontiers in Medicine·Mona El ZarifNehman Makdissy
Nov 10, 2020·Experimental Eye Research·Jorge L Alió Del BarrioJorge L Alió
Apr 23, 2021·Small·Sadjad KhosravimelalMazaher Gholipourmalekabadi
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wan-Hsin ChangYuan-Hao Howard Hsu