Adipose mesenchymal stromal cells response to ionizing radiation

Cytotherapy
Osama Muhammad MariaThierry Muanza

Abstract

This study evaluates the biological response of adipose tissue-derived mesenchymal stromal cells (aMSCs) to ionizing radiation (IR). Irradiated BALB/c mice aMSCs were characterized for functionality and phenotype. The clonogenic capacity of irradiated aMSCs was assessed and compared with those of metastatic breast cancer cell line (4T1) and normal mouse fibroblasts (NIH3T3-wt). We investigated the IR-induced DNA damage response, apoptosis, changes in cell cycle (CC) dynamics and protein and gene expression. Irradiated and non-irradiated aMSCs were able to differentiate into adipocytes, chondrocytes and osteocytes with no significant difference. Irradiated aMSCs maintained the expression of mesenchymal stromal cells (MSCs) surface antigens and, as expected, were negative for hematopoietic stem cells (HSCs) surface antigens when tested up to 7 days after IR for all irradiation doses with no significant difference. Clonogenically, irradiated aMSCs had higher relative survival fraction and plating efficiency than 4T1 and NIH3T3-wt. Irradiated aMSCs expressed higher □H2AX and significantly showed faster and more time-efficient IR-induced DNA damage response evident by up-regulated DNA-PKcs and RAD51. Two hours after IR, most of aMSC...Continue Reading

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Citations

Sep 14, 2018·International Journal of Molecular Sciences·Kateryna ShrederClaudia Fournier
Jul 11, 2018·International Journal of Molecular Sciences·Annemarie BaaßeGuido Hildebrandt
Dec 17, 2019·Dose-response : a Publication of International Hormesis Society·Ningning HeQiang Liu
Jan 1, 2021·Frontiers in Cell and Developmental Biology·Marina KonkovaSvetlana Kostyuk

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