Adipose stem cell sheets improved cardiac function in the rat myocardial infarction, but did not alter cardiac contractile responses to β-adrenergic stimulation

Biomedical Research
Yuki OtsukiMotonobu Nishimura

Abstract

Adipose stem cells (ASCs) are a source of regenerative cells available for autologous transplantation to hearts. We compared protective actions of ASC sheets on rat myocardial infarction (MI) in comparison with those of skeletal myoblast cell sheets. Their effects on infarcted hearts were evaluated by biological, histochemical as well as physiological analyses. ASC sheets secreted higher concentrations of angiogenic factors (HGF, VEGF, and bFGF; P < 0.05) under normoxic and hypoxic conditions than those of myoblast cell sheets, associated with reduction of cell apoptosis (P < 0.05). Like myoblast cell sheets, ASC sheets improved cardiac function (P < 0.05) and decreased the plasma level of ANP (P < 0.05) in MI hearts. ASC sheets restored cardiac remodeling characterized by fibrosis, cardiac hypertrophy and impaired angiogenesis (P < 0.05), which was associated with increases in angiogenic factors (P < 0.05). In isolated perfused rat hearts, ASC sheets improved both systolic and diastolic functions, which was comparable to cardiac functions of myoblast cell sheets, while both cell sheets failed to restore cardiac contractile response to either isoproterenol, pimobendan or dibutyryl cAMP. These results indicated that ASC sheets i...Continue Reading

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Citations

Jul 1, 2017·Tissue Engineering. Part B, Reviews·Panithi SukhoYvonne M Bastiaansen-Jenniskens
Jul 29, 2017·Current Cardiology Reports·Buntaro Fujita, Wolfram-Hubertus Zimmermann
Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Feb 23, 2020·International Journal of Molecular Sciences·Loubna MaziniGabriel Malka
Sep 19, 2019·Circulation Journal : Official Journal of the Japanese Circulation Society·Maya AdachiIchiro Hisatome
Mar 16, 2021·Circulation Journal : Official Journal of the Japanese Circulation Society·Mai WatanabeIchiro Hisatome
Nov 18, 2021·Bioengineered·Li ZengSheng-Kang Luo

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