Adipose stem cells from type 2 diabetic mice exhibit therapeutic potential in wound healing.

Stem Cell Research & Therapy
Yongfa SunXiao Dong

Abstract

Diabetic patients suffer from impaired wound healing. Mesenchymal stem cell (MSC) therapy represents a promising approach toward improving skin wound healing through the release of soluble growth factors and cytokines that stimulate new vessel formation and modulate inflammation. Whether adipose tissue-derived MSCs (ASCs) from type 2 diabetes (T2D) donors are suitable for skin damage repair remains largely unknown. In this study, we compared the phenotype and functionality of ASCs harvested from high-fat diet (HFD) and streptozotocin (STZ)-induced T2D or control mice, and assessed their abilities to promote wound healing in an excisional wound splinting mouse model with T2D. T2D ASCs expressed similar cellular markers as control ASCs but secreted less hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and transforming growth factor β (TGF-β). T2D ASCs were somewhat less effective in promoting healing of the wound, as manifested by slightly reduced re-epithelialization, cutaneous appendage regeneration, and collagen III deposition in wound tissues. In vitro, T2D ASCs promoted proliferation and migration of skin fibroblasts to a comparable extent as control ASCs via suppression of inflammation and macropha...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Dec 25, 2002·Microscopy Research and Technique·Jie LiRobert S Kirsner
Nov 18, 2005·Lancet·Vincent Falanga
Jun 23, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Mi Hyang MoonJin Sup Jung
Sep 10, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Yaojiong WuEdward E Tredget
Dec 8, 2007·European Biophysics Journal : EBJ·Laura J VellaAndrew F Hill
May 16, 2008·Nature·Geoffrey C GurtnerMichael T Longaker
May 16, 2009·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Hiroshi Mizuno
Sep 23, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Danielle T Loughlin, Carol M Artlett
Apr 13, 2010·Stem Cells and Development·Christopher CramerReza Izadpanah
Jul 29, 2010·Seminars in Liver Disease·Thomas A Wynn, Luke Barron
Oct 15, 2011·Nature Reviews. Immunology·Peter J Murray, Thomas A Wynn
Nov 19, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Zhifeng HuangXiaokun Li
Oct 12, 2012·Cellular and Molecular Life Sciences : CMLS·Kirsten A BielefeldBenjamin A Alman
May 1, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Francesca CianfaraniTeresa Odorisio
Aug 29, 2013·Journal of Biomedical Nanotechnology·E Biazar, S Heidari Keshel
Apr 26, 2015·Biochemical and Biophysical Research Communications·In-Su ParkJin Chul Ahn
Sep 12, 2015·Current Medical Research and Opinion·Anthony H BarnettSanjay Kalra
Dec 3, 2016·Experimental Biology and Medicine·Ignazio BarbagalloLuca Vanella
Jun 29, 2017·Molecular Medicine Reports·Dayong ZhangJianping Pan
Jul 15, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mengmeng WangHongjun Wang

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Methods Mentioned

BETA
flow cytometry
Assay
PCR
ELISA

Software Mentioned

ImageJ
CellQuest

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