Adipose stroma induces branching morphogenesis of engineered epithelial tubules.

Tissue Engineering. Part a
Amira L PavlovichCeleste M Nelson

Abstract

The mammary gland and other treelike organs develop their characteristic fractal geometries through branching morphogenesis, a process in which the epithelium bifurcates and invades into the surrounding stroma. Controlling the pattern of branching is critical for engineering these organs. In vivo, the branching process is instructed by stromal-epithelial interactions and adipocytes form the largest component of the fatty stroma that surrounds the mammary epithelium. Here, we used microlithographic approaches to engineer a three-dimensional culture model that enables analysis of the effect of adipocytes on the pattern of branching morphogenesis of mammary epithelial cells. We found that adipocyte-rich stroma induces branching through paracrine signals, including hepatocyte growth factor, but does not affect the branching pattern per se. This tissue engineering approach can be expanded to other organs, and should enable piecemeal analysis of the cellular populations that control patterning during normal development.

References

Nov 1, 1992·Proceedings of the Society for Experimental Biology and Medicine·J C CalvoD Rodbard
Dec 1, 1989·Differentiation; Research in Biological Diversity·J L Rodríguez Fernández, A Ben-Ze'ev
May 1, 1985·The Journal of Cell Biology·J F Levine, F E Stockdale
Mar 1, 1984·Experimental Cell Research·J F Levine, F E Stockdale
May 1, 1982·Developmental Biology·T SakakuraY Nishizuka
Jan 1, 1994·Annual Review of Nutrition·P CorneliusM D Lane
Jun 25, 1998·In Vitro Cellular & Developmental Biology. Animal·R C HoveyT B McFadden
May 20, 2000·Journal of Mammary Gland Biology and Neoplasia·J V SorianoR Montesano
Mar 29, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christine CouldreyJeffrey Green
Aug 31, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Matthew J Naylor, Christopher J Ormandy
Sep 1, 1963·The Journal of Cell Biology·L NAPOLITANO
Jul 17, 2004·Annual Review of Biomedical Engineering·Charles W Patrick
Sep 24, 2004·Experimental Cell Research·Claudia FischbachTorsten Blunk
Nov 19, 2004·Nature·Neil A BhowmickHarold L Moses
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Celeste M Nelson, Mina J Bissell
Aug 19, 2006·Differentiation; Research in Biological Diversity·Mark D SternlichtZena Werb
Sep 15, 2006·Current Opinion in Biotechnology·Celeste M Nelson, Joe Tien
Sep 19, 2006·Current Opinion in Biotechnology·Salman R Khetani, Sangeeta N Bhatia
Feb 9, 2007·Differentiation; Research in Biological Diversity·Sandeep DayaHilary A Macqueen
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Chih-Chao YangKaren J L Burg
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hidetoshi MoriCeleste M Nelson
Oct 24, 2009·Developmental Biology·Jennifer N Lilla, Zena Werb
Nov 26, 2009·Cell·Kandice R LeventalValerie M Weaver
Mar 10, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·I LeventalP A Janmey

❮ Previous
Next ❯

Citations

Aug 11, 2011·Nature Reviews. Molecular Cell Biology·Nikolce Gjorevski, Celeste M Nelson
Sep 17, 2011·Tissue Engineering. Part a·Silva KrauseMaricel V Maffini
Nov 2, 2012·Stem Cell Research & Therapy·Sriram Manivannan, Celeste M Nelson
Dec 4, 2013·Advanced Drug Delivery Reviews·Maria HåkansonMirren Charnley
May 1, 2013·Biomatter·Wenting Zhu, Celeste M Nelson
Jan 28, 2015·Endocrine-related Cancer·Denise K ReavesJodie M Fleming
Oct 26, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Hyung-Do Kim, Shelly R Peyton
Dec 23, 2016·Cellular and Molecular Life Sciences : CMLS·Bryan A NergerCeleste M Nelson
Aug 4, 2021·Reproductive Toxicology·Thalles Fernando Rocha RuizEllen Cristina Rivas Leonel

❮ Previous
Next ❯

Methods Mentioned

BETA
rheometry
PCR

Software Mentioned

Photoshop
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
N W ShappellB B Asch
Integrative Biology : Quantitative Biosciences From Nano to Macro
Mirren CharnleyMatthias P Lutolf
Current Opinion in Genetics & Development
William P Daley, K M Yamada
© 2021 Meta ULC. All rights reserved