Adipose tissue beta-adrenergic and A1 adenosine receptors in suckling pigs

Journal of Animal Science
H J MersmannE O Smith

Abstract

During the first few weeks after birth, major changes occur in porcine adipocyte lipid metabolism. Two of the important receptors controlling lipid metabolism in adipocytes are the beta-adrenergic receptors (betaAR) and the A1 adenosine receptors (A1R). To gain insight into the role of these receptors in modulating neonatal adipocyte lipid metabolism, we measured receptor affinity and number in suckling pigs. Adipose tissue from crossbred (X-Bred) and genetically obese suckling pigs at 0, 3, 10, and 17 d of age was used to prepare crude membranes. The betaAR and A1R number and affinity were measured in membranes by equilibrium saturation binding with radioligands. Obese pigs were smaller than X-Bred pigs (average weight = 1.62 and 2.43 kg for obese and X-Bred, respectively; P < .01). Osmium-fixed adipocytes were larger in obese pigs than in X-Bred pigs (average cell diameter = 34.4 and 30.1 microm for obese and X-Bred, respectively; P < .01). In the obese and X-Bred pigs, the affinity of the betaAR for iodocyanopindolol was greater (lower Kd) at 17 d than at the younger ages (average Kd = 177 pM at 17 d compared with > 330 pM at younger ages; age effect P < .01). The pattern for the betaAR number was complex; the lowest recepto...Continue Reading

Citations

Dec 19, 2017·Frontiers in Pharmacology·Marco Tozzi, Ivana Novak
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Feb 23, 2021·Frontiers in Neuroscience·Lachlan Van SchaikJoseph A Rathner
Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R L McNeelH J Mersmann
Sep 11, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S T DingH J Mersmann

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