Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota

Nature Communications
Lucie GeurtsPatrice D Cani

Abstract

Obesity is a pandemic disease associated with many metabolic alterations and involves several organs and systems. The endocannabinoid system (ECS) appears to be a key regulator of energy homeostasis and metabolism. Here we show that specific deletion of the ECS synthesizing enzyme, NAPE-PLD, in adipocytes induces obesity, glucose intolerance, adipose tissue inflammation and altered lipid metabolism. We report that Napepld-deleted mice present an altered browning programme and are less responsive to cold-induced browning, highlighting the essential role of NAPE-PLD in regulating energy homeostasis and metabolism in the physiological state. Our results indicate that these alterations are mediated by a shift in gut microbiota composition that can partially transfer the phenotype to germ-free mice. Together, our findings uncover a role of adipose tissue NAPE-PLD on whole-body metabolism and provide support for targeting NAPE-PLD-derived bioactive lipids to treat obesity and related metabolic disorders.

References

Nov 13, 2001·British Journal of Pharmacology·N Jamshidi, D A Taylor
May 3, 2005·The Journal of Clinical Investigation·Kathryn E Wellen, Gökhan S Hotamisligil
Apr 26, 2007·Diabetes·Patrice D CaniRémy Burcelin
Jul 17, 2007·Neuropharmacology·Jie LiuGeorge Kunos
Sep 19, 2007·British Journal of Pharmacology·E RybergP J Greasley
Jan 10, 2009·Obesity·Laurence HoareauRégis Roche
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 27, 2009·The Journal of Biological Chemistry·Ahmed EssaghirJean-Baptiste Demoulin
May 6, 2009·Biochemical Pharmacology·Harald S Hansen, Thi Ai Diep
Sep 3, 2009·Molecular and Cellular Endocrinology·Sandra GalicGregory R Steinberg
Mar 23, 2010·Drug Discovery Today·Giulio G Muccioli
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jul 29, 2010·Molecular Systems Biology·Giulio G MuccioliPatrice D Cani
Aug 1, 2012·Nature Reviews. Endocrinology·Henrike SellJuergen Eckel
Oct 18, 2012·American Journal of Physiology. Endocrinology and Metabolism·Samreen K SyedAlexander M Efanov
Nov 30, 2012·Molecular Endocrinology·Shannon E MullicanMitchell A Lazar
Jan 17, 2013·Diabetes·Kevin Y LeeC Ronald Kahn
Jun 27, 2013·The Journal of Biological Chemistry·Cristoforo SilvestriVincenzo Di Marzo
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li YeBruce M Spiegelman
Jul 9, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Resat CinarGeorge Kunos
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mireille AlhouayekGiulio G Muccioli
Oct 22, 2013·Diabetes & Metabolism·M Lafontan

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Citations

Jan 26, 2016·American Journal of Physical Anthropology·Katherine R Amato
Dec 19, 2015·Nature Reviews. Endocrinology·Patrice D CaniAmandine Everard
Jan 26, 2016·Trends in Biochemical Sciences·Ilaria PettinatiChristopher J Schofield
Jul 24, 2015·Diabetologia·José María Moreno-NavarreteJosé Manuel Fernández-Real
Jul 8, 2015·Clinical Biochemistry·Irina A KirpichCraig J McClain
Aug 24, 2016·Scientific Reports·Anne DrougardClaude Knauf
Jan 16, 2016·Journal of Lipid Research·Lucia M KrottVincenzo Di Marzo
Jul 5, 2017·Hormone Molecular Biology and Clinical Investigation·Yi-Wei Lin, Li-Na Wei
Sep 7, 2017·American Journal of Physiology. Endocrinology and Metabolism·Matthias Van HulPatrice D Cani
Jun 10, 2017·Journal of Lipid Research·Zhongyi ChenSean S Davies
Dec 1, 2017·Current Pathobiology Reports·Benedicte F JordanPatrice D Cani
Aug 18, 2018·Nature Reviews. Drug Discovery·Vincenzo Di Marzo
Oct 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Petteri RinneSabine Steffens
May 31, 2016·F1000Research·Arnau Busquets GarciaGiovanni Marsicano
May 14, 2019·Endocrine Reviews·Marialetizia RastelliClaude Knauf
Aug 27, 2019·Current Pharmaceutical Design·Fabiana Piscitelli, Cristoforo Silvestri
Sep 11, 2019·Endocrinology·Matthew D LynesYu-Hua Tseng
Dec 14, 2019·Nature Reviews. Neurology·Luigia CristinoVincenzo Di Marzo
Dec 24, 2019·Clinical and Experimental Pharmacology & Physiology·Helda TutunchiAlireza Ostadrahimi
Feb 29, 2020·International Journal of Molecular Sciences·Nicola ForteLuigia Cristino
Aug 11, 2020·American Journal of Physiology. Endocrinology and Metabolism·Marialetizia RastelliPatrice D Cani
Nov 7, 2019·Current Diabetes Reports·Alain VeilleuxCristoforo Silvestri
Aug 23, 2019·Nutrients·Vincenzo Di Marzo, Cristoforo Silvestri
Feb 15, 2019·Frontiers in Endocrinology·Céline Gérard, Hubert Vidal
Jul 19, 2019·Frontiers in Endocrinology·Liyuan ZhouMingqun Deng
Nov 30, 2019·Reviews in Endocrine & Metabolic Disorders·José María Moreno-Navarrete, José Manuel Fernandez-Real

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Datasets Mentioned

BETA
GSE56852

Methods Mentioned

BETA
NMR
PCR
ELISA
chips

Software Mentioned

Napepld
GraphPad
Affymetrix Gene Expression Console
iTOL
uclust
Affymetrix APT suite
DAVID
ImageJ
GraphPad Prism
QIIME

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