Adipose triglyceride lipase is involved in the mobilization of triglyceride and retinoid stores of hepatic stellate cells

Biochimica Et Biophysica Acta
Ulrike TaschlerRobert Zimmermann

Abstract

Hepatic stellate cells (HSCs) store triglycerides (TGs) and retinyl ester (RE) in cytosolic lipid droplets. RE stores are degraded following retinoid starvation or in response to pathogenic stimuli resulting in HSC activation. At present, the major enzymes catalyzing lipid degradation in HSCs are unknown. In this study, we investigated whether adipose triglyceride lipase (ATGL) is involved in RE catabolism of HSCs. Additionally, we compared the effects of ATGL deficiency and hormone-sensitive lipase (HSL) deficiency, a known RE hydrolase (REH), on RE stores in liver and adipose tissue. We show that ATGL degrades RE even in the presence of TGs, implicating that these substrates compete for ATGL binding. REH activity was stimulated and inhibited by comparative gene identification-58 and G0/G1 switch gene-2, respectively, the physiological regulators of ATGL activity. In cultured primary murine HSCs, pharmacological inhibition of ATGL, but not HSL, increased RE accumulation. In mice globally lacking ATGL or HSL, RE contents in white adipose tissue were decreased or increased, respectively, while plasma retinol and liver RE levels remained unchanged. In conclusion, our study shows that ATGL acts as REH in HSCs promoting the degrada...Continue Reading

References

Nov 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M YamadaD S Goodman
Jun 15, 1994·The Biochemical Journal·G TrøenR Blomhoff
Jan 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W F SeifertD L Knook
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J OsugaN Yamada
Nov 22, 2001·The Journal of Biological Chemistry·Guenter HaemmerleRudolf Zechner
Jan 31, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karine HellemansAlbert Geerts
Nov 20, 2004·Science·Robert ZimmermannRudolf Zechner
Feb 26, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zili DanFelix Stickel
Apr 14, 2005·Nutrition Reviews·George Wolf
Jun 16, 2005·The Journal of Investigative Dermatology·Jay Gao, Marica Simon
May 12, 2006·Journal of Neurobiology·Rune Blomhoff, Heidi Kiil Blomhoff
Nov 1, 2006·The Journal of Biological Chemistry·Martina SchweigerRobert Zimmermann
Jul 3, 2007·Biochemical and Biophysical Research Communications·Jay G Gao, Marcia Simon
Jul 5, 2007·Journal of Proteome Research·René BartzPingsheng Liu
Jul 22, 2008·Biochemical and Biophysical Research Communications·Tommaso MelloSonal P Sanghani
Dec 17, 2008·Biochimica Et Biophysica Acta·William S BlanerJenny Libien
Feb 28, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kristoffer StrömCecilia Holm
Apr 30, 2009·American Journal of Physiology. Endocrinology and Metabolism·Martina SchweigerRudolf Zechner
Sep 3, 2009·Journal of Lipid Research·Renate SchreiberAchim Lass
Apr 29, 2010·The Journal of Biological Chemistry·Prakash G ChandakDagmar Kratky
Oct 23, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kuok Teong OngDouglas G Mashek
Feb 26, 2011·Nutrients·Diana N D'AmbrosioWilliam S Blaner
Mar 4, 2011·Current Medicinal Chemistry·P M AmannA V Bazhin
Apr 6, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jiang Wei WuGrant A Mitchell
Apr 19, 2011·Best Practice & Research. Clinical Gastroenterology·Ursula E Lee, Scott L Friedman
May 19, 2011·Biochimica Et Biophysica Acta·Renate SchreiberAchim Lass
Jun 7, 2011·Cell Metabolism·Maryam AhmadianHei Sook Sul
Jul 19, 2011·Biochimica Et Biophysica Acta·Yohei ShirakamiWilliam S Blaner
Aug 2, 2011·Chemico-biological Interactions·Elisabetta MormoneNatalia Nieto
Mar 13, 2012·Cell Metabolism·Rudolf ZechnerFrank Madeo
Sep 5, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Pooja JhaMichael Trauner
Oct 8, 2013·Nature Chemical Biology·Nicole MayerRolf Breinbauer
Jan 21, 2014·Biochimica Et Biophysica Acta·Harald M NagyRobert Zimmermann
Mar 29, 2014·Human Molecular Genetics·Carlo PirazziStefano Romeo

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Citations

Dec 8, 2015·Experimental Cell Research·Wen-Jun ShenFredric B Kraemer
Mar 1, 2016·Biochimica Et Biophysica Acta·Christoph Heier, Guenter Haemmerle
Jul 15, 2015·Cellular and Molecular Life Sciences : CMLS·Thomas Oliver Eichmann, Achim Lass
Mar 12, 2017·Nutrients·Patrick Borel, Charles Desmarchelier
Sep 4, 2015·Journal of Lipid Research·Thomas O EichmannAchim Lass
May 15, 2016·Journal of Lipid Research·Maidina TuohetahuntilaJ Bernd Helms
Dec 30, 2017·Nutrients·Ali SaeedKlaas Nico Faber
Dec 22, 2019·Cells·Olga KhomichBirke Bartosch
Mar 14, 2020·The Biochemical Journal·Alexander Yang, Emilio P Mottillo
Oct 7, 2015·The Journal of Clinical Endocrinology and Metabolism·Marketa KovarovaAndreas Peter
May 9, 2019·Genesis : the Journal of Genetics and Development·Suya Wang, Alexander R Moise
May 1, 2020·Journal of Lipid Research·Gernot F GrabnerRobert Zimmermann
Dec 31, 2015·Chembiochem : a European Journal of Chemical Biology·Carina DolerRolf Breinbauer
Oct 11, 2020·Cells·Martijn R MolenaarJ Bernd Helms
Nov 25, 2020·Cell Metabolism·Parth TrivediScott L Friedman
Feb 28, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Maya W HaakerJ Bernd Helms
May 4, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Carina WagnerAchim Lass
Feb 9, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Igor O ShmarakovWilliam S Blaner

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Methods Mentioned

BETA
electrophoresis
Fluorescence
FCS
protein assay

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