Mar 1, 1989

Adjacent interval analysis distinguishes among gating mechanisms for the fast chloride channel from rat skeletal muscle

The Journal of Physiology
A L Blatz, K L Magleby

Abstract

1. The durations of adjacent open and shut intervals, obtained with the patch-clamp technique from fast Cl- channels in tissue-cultured rat skeletal muscle, were analysed to distinguish among eight previously considered gating mechanisms for the channel which differed in the connections among the states. 2. Open intervals were separated into groups based on the duration of the shut intervals which occurred before or after each open interval. Fitting these conditional open distributions with sums of exponentials indicated that they were described by two exponential components. 3. The time constants of the two components in the conditional open distributions were independent of the adjacent shut interval durations. The observation of invariant time constants is consistent with gating mechanisms in which the rate constants for transitions among the states remain constant with time (discrete Markov process). 4. In contrast to the invariant time constants, the areas of the two components in the conditional open distributions were dependent on the adjacent shut interval durations. The area of the fast open component increased, and the area of the slow open component decreased, as the duration of adjacent shut intervals increased. Thu...Continue Reading

  • References
  • Citations12

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations12

Citations

Mentioned in this Paper

Chloride Ion Level
Biochemical Pathway
Patch-Clamp Techniques
Resting Potentials
PDZK1 gene
Ion Channel
Rat Skeletal Muscle
Clamping Activity
Muscle
Chloride Channels

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.