Adjacent interval analysis distinguishes among gating mechanisms for the fast chloride channel from rat skeletal muscle.

The Journal of Physiology
A L Blatz, K L Magleby

Abstract

1. The durations of adjacent open and shut intervals, obtained with the patch-clamp technique from fast Cl- channels in tissue-cultured rat skeletal muscle, were analysed to distinguish among eight previously considered gating mechanisms for the channel which differed in the connections among the states. 2. Open intervals were separated into groups based on the duration of the shut intervals which occurred before or after each open interval. Fitting these conditional open distributions with sums of exponentials indicated that they were described by two exponential components. 3. The time constants of the two components in the conditional open distributions were independent of the adjacent shut interval durations. The observation of invariant time constants is consistent with gating mechanisms in which the rate constants for transitions among the states remain constant with time (discrete Markov process). 4. In contrast to the invariant time constants, the areas of the two components in the conditional open distributions were dependent on the adjacent shut interval durations. The area of the fast open component increased, and the area of the slow open component decreased, as the duration of adjacent shut intervals increased. Thu...Continue Reading

Citations

Dec 1, 1992·Mathematical Biosciences·F G Ball, J A Rice
Aug 1, 1991·Journal of Bioenergetics and Biomembranes·O B McManus
Jan 13, 2006·British Journal of Pharmacology·David Colquhoun
Apr 23, 2008·Current Protocols in Neuroscience·M B Jackson
Jun 20, 2018·The Journal of Physiology·Alasdair J GibbStephen F Traynelis
Aug 22, 1992·Proceedings. Biological Sciences·K L Magleby, L Song
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·S R Vaccaro
Mar 5, 2015·Current Protocols in Pharmacology·Chris Shelley

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