Mar 1, 1989

Adjacent interval analysis distinguishes among gating mechanisms for the fast chloride channel from rat skeletal muscle

The Journal of Physiology
A L Blatz, K L Magleby


1. The durations of adjacent open and shut intervals, obtained with the patch-clamp technique from fast Cl- channels in tissue-cultured rat skeletal muscle, were analysed to distinguish among eight previously considered gating mechanisms for the channel which differed in the connections among the states. 2. Open intervals were separated into groups based on the duration of the shut intervals which occurred before or after each open interval. Fitting these conditional open distributions with sums of exponentials indicated that they were described by two exponential components. 3. The time constants of the two components in the conditional open distributions were independent of the adjacent shut interval durations. The observation of invariant time constants is consistent with gating mechanisms in which the rate constants for transitions among the states remain constant with time (discrete Markov process). 4. In contrast to the invariant time constants, the areas of the two components in the conditional open distributions were dependent on the adjacent shut interval durations. The area of the fast open component increased, and the area of the slow open component decreased, as the duration of adjacent shut intervals increased. Thu...Continue Reading

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Mentioned in this Paper

Chloride Ion Level
Biochemical Pathway
Patch-Clamp Techniques
Resting Potentials
PDZK1 gene
Ion Channel
Rat Skeletal Muscle
Clamping Activity
Chloride Channels

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