Adjustable under-expression of yeast mating pathway proteins in Saccharomyces cerevisiae using a programmed ribosomal frameshift

Applied Microbiology and Biotechnology
Min-Yeon Choi, Sang-Hyun Park

Abstract

Experimental research in molecular biology frequently relies on the promotion or suppression of gene expression, an important tool in the study of its functions. Although yeast is among the most studied model systems with the ease of maintenance and manipulation, current experimental methods are mostly limited to gene deletion, suppression or overexpression of genes. Therefore, the ability to reduce protein expressions and then observing the effects would promote a better understanding of the exact functions and their interactions. Reducing protein expression is mainly limited by the difficulties associated with controlling the reduction level, and in some cases, the initial endogenous abundance is too low. For the under-expression to be useful as an experimental tool, repeatability and stability of reduced expression is important. We found that cis-elements in programmed -1 ribosomal frameshifting (-1RFS) of beet western yellow virus (BWYV) could be utilized to reduced protein expression in Saccharomyces cerevisiae. The two main advantages of using -1RFS are adjustable reduction rates and ease of use. To demonstrate the utility of this under-expression system, examples of reduced protein abundance were shown using yeast mating...Continue Reading

References

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E A ElionG R Fink
Jul 28, 1995·The Journal of Biological Chemistry·R J DohmenA Varshavsky
Jan 1, 1996·Annual Review of Genetics·P J Farabaugh
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y G KimA Rich
Mar 4, 2000·Progress in Nucleic Acid Research and Molecular Biology·P J Farabaugh
Jan 5, 2002·Methods in Enzymology·Ginger A HoffmanHenrik G Dohlman
Apr 19, 2002·Annals of the New York Academy of Sciences·Francisco H AndradeJohn D Porter
Apr 11, 2003·Nature·William J BlakeJ J Collins
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Mar 20, 2004·Developmental Biology·Junying YuRichard M Schultz
Dec 1, 2004·Annual Review of Genetics·Monica A Schwartz, Hiten D Madhani
Apr 11, 2006·Trends in Biochemical Sciences·Leon O Murphy, John Blenis
Aug 2, 2006·Molecular BioSystems·Ulrike S EggertTimothy J Mitchison
Apr 26, 2007·Applied Microbiology and Biotechnology·Achim Aigner
May 19, 2007·Nature Reviews. Genetics·Charles BooneBrenda J Andrews
Oct 29, 2008·Current Opinion in Microbiology·Hitoshi Nakayashiki, Quoc Bao Nguyen
Apr 14, 2009·Nature·Sabrina L SpencerPeter K Sorger
Jan 19, 2010·Biochimica Et Biophysica Acta·Eun Kyung Kim, Eui-Ju Choi
Nov 15, 2011·Molecules and Cells·Jae Young HurSang-Hyun Park
Feb 16, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·László Bodai, J Lawrence Marsh
Mar 8, 2013·Open Biology·Laura MerliniSophie G Martin
Apr 25, 2013·Applied Microbiology and Biotechnology·Yasushi KamisakaMasakazu Yamaoka
Dec 7, 2013·Biochemical and Biophysical Research Communications·Eunyeong Shim, Sang-Hyun Park
Jun 6, 2015·Cell·Yolanda T ChongBrenda J Andrews

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