PMID: 9636162Jun 24, 1998Paper

Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins

Proceedings of the National Academy of Sciences of the United States of America
P ZávodszkyG A Petsko

Abstract

3-Isopropylmalate dehydrogenase (IPMDH, E.C. 1.1.1.85) from the thermophilic bacterium Thermus thermophilus HB8 is homologous to IPMDH from the mesophilic Escherichia coli, but has an approximately 17 degreesC higher melting temperature. Its temperature optimum is 22-25 degreesC higher than that of the E. coli enzyme; however, it is hardly active at room temperature. The increased conformational rigidity required to stabilize the thermophilic enzyme against heat denaturation might explain its different temperature-activity profile. Hydrogen/deuterium exchange studies were performed on this thermophilic-mesophilic enzyme pair to compare their conformational flexibilities. It was found that Th. thermophilus IPMDH is significantly more rigid at room temperature than E. coli IPMDH, whereas the enzymes have nearly identical flexibilities under their respective optimal working conditions, suggesting that evolutionary adaptation tends to maintain a "corresponding state" regarding conformational flexibility. These observations confirm that conformational fluctuations necessary for catalytic function are restricted at room temperature in the thermophilic enzyme, suggesting a close relationship between conformational flexibility and enzy...Continue Reading

References

Jan 1, 1979·Advances in Protein Chemistry·P L Privalov
Aug 23, 1979·Nature·P L Privalov, T N Tsalkova
Dec 13, 1977·Biochemistry·M R Eftink, C A Ghiron
Aug 1, 1975·CRC Critical Reviews in Biochemistry·G CareriE Gratton
Dec 18, 1991·European Journal of Biochemistry·R Jaenicke
Aug 1, 1990·FEBS Letters·R Jaenicke, P Závodszky
Jan 1, 1988·Methods in Enzymology·G B Kohlhaw
Dec 1, 1987·Protein Engineering·M Vihinen
Jan 1, 1972·Methods in Enzymology·H Susi
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Jan 1, 1984·Progress in Biophysics and Molecular Biology·A Cooper
Oct 29, 1982·Molecular and Cellular Biochemistry·C WoodwardE Tüchsen
Sep 1, 1993·Proteins·Y BaiS W Englander

❮ Previous
Next ❯

Citations

Jun 27, 2007·Chembiochem : a European Journal of Chemical Biology·Dieter Perl, Franz Xaver Schmid
Jun 26, 2012·Pflügers Archiv : European journal of physiology·Elena BossiAntonio Peres
Dec 13, 2006·Extremophiles : Life Under Extreme Conditions·Georges Feller
Jan 18, 2006·Journal of Molecular Modeling·V Raghu Ram Malapaka, Brian C Tripp
Jan 20, 2007·Journal of Molecular Modeling·Vojtech SpiwokBlanka Králová
Dec 30, 2006·Marine Biotechnology·J-C MarxC Gerday
Sep 19, 2009·Applied Biochemistry and Biotechnology·Om Prakash, Nivedita Jaiswal
Jul 20, 2010·Applied Biochemistry and Biotechnology·Hai-Yan ZhouYong-Yao Wu
Jun 14, 2005·Biochimica Et Biophysica Acta·Moeava Tehei, Giuseppe Zaccai
Dec 19, 2003·Protein Expression and Purification·Gudrún JónsdóttirAgústa Gudmundsdóttir
Mar 14, 2003·Journal of Molecular Biology·Patrick L WintrodeWilliam A Goddard
Apr 15, 2003·Journal of Molecular Biology·Tony CollinsGeorges Feller
Jun 8, 2001·Progress in Biophysics and Molecular Biology·P Chakrabarti, D Pal
Jun 23, 2000·Enzyme and Microbial Technology·J B HaukssonB Ásgeirsson
Feb 9, 2000·Pharmacology & Therapeutics·G K Farber
May 2, 2002·Biochimica Et Biophysica Acta·Patrizia Cioni, Giovanni B Strambini
Feb 17, 2000·Trends in Biotechnology·C GerdayG Feller
Jun 27, 2000·Journal of Biotechnology·R Jaenicke
Oct 12, 2000·Biophysical Chemistry·M Brunori
Sep 23, 2003·Biophysical Chemistry·Dagmar Ringe, Gregory A Petsko
Jan 23, 1999·Current Opinion in Structural Biology·R Jaenicke, G Böhm
Feb 13, 2001·Trends in Biochemical Sciences·F H ArnoldA Gershenson
Jun 26, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·P A Fields
Feb 18, 1999·Current Opinion in Chemical Biology·D W Hough, M J Danson
Oct 20, 2005·Journal of Bioscience and Bioengineering·Anne HoyouxCharles Gerday
Sep 29, 2007·Biochemistry·Lea V MichelKara L Bren
Mar 19, 2011·Biomacromolecules·Cesar A GodoyFernando López-Gallego
May 11, 2006·Chemical Reviews·Mats H M OlssonArieh Warshel
Aug 23, 2011·Journal of Agricultural and Food Chemistry·Li-Chiun LeeJei-Fu Shaw
Feb 14, 2009·Journal of Medicinal Chemistry·Douglas S AuldJames Inglese

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.