Adjuvant effect of Achyranthes bidentata saponins on specific antibody and cellular response to ovalbumin in mice

Vaccine
Hongxiang Sun

Abstract

In this study, the haemolytic activities of Achyranthes bidentata saponins (ABS) and its adjuvant potentials on the cellular and humoral immune responses of ICR mice against ovalbumin (OVA) were evaluated. We determined the haemolytic activity of ABS using 0.5% rabbit red blood cell. The concentration inducing 50% of the maximum haemolysis (HD50) for ABS was 164.59+/-13.41 microg/ml. ICR mice were immunized subcutaneously with OVA 100 microg alone or with OVA 100 microg dissolved in saline containing Alum (200 microg), QuilA (10 and 20 microg) or ABS (50, 100 or 200 microg) on Days 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS)- and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. ABS significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice especially at a dose of 100 microg (P<0.05 or P<0.025). OVA-specific serum IgG, IgG1 and IgG2b antibody titers were also significantly enhanced by ABS compared with OVA control group (P<0.05 or P<0.025). The results suggest that ABS showed a slight haemolytic effect and enhanced significantly a specific antibody and cellular response against OVA in mice.

Citations

Dec 3, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tao SunDayun Zhao
Feb 27, 2007·Journal of Zhejiang University. Science. B·Zahid Iqbal RajputAbdullah G Arijo
Oct 7, 2009·Expert Review of Vaccines·Carlos Gamazo, Juan Manuel Irache
Dec 1, 2009·Journal of Ethnopharmacology·Cui-Cui HeJian-Xin Li
Feb 12, 2009·Vaccine·Hong-Xiang SunYi-Ping Ye
Jan 19, 2012·Chemistry & Biodiversity·Camila Rodrigues AdãoJosé Paz Parente
Jun 30, 2009·Vaccine·Xiaoming Song, Songhua Hu
May 12, 2012·International Journal of Biological Macromolecules·Zhang YuqingHu Yuanliang
Aug 28, 2016·Biochemical and Biophysical Research Communications·Sungwon LeeKyungjae Kim
Nov 26, 2016·Bioscience, Biotechnology, and Biochemistry·Youngjoo LeeKyungjae Kim
Apr 29, 2014·Expert Review of Vaccines·Sergio Rosales-Mendoza, Jorge A Salazar-González

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.