Adjuvant effect of the Propionibacterium acnes and its purified soluble polysaccharide on the immunization with plasmidial DNA containing a Trypanosoma cruzi gene

Microbiology and Immunology
Juliana Sekeres MussalemIeda Longo-Maugéri

Abstract

In the present work we investigated the role of killed Propionibacterium acnes or a soluble polysaccharide extracted from bacterium cell wall in modulated experimental immunization with plasmidial DNA. We used a plasmid, p154/13, containing a gene-encoding catalytic domain of Trypanosoma cruzi (T. cruzi) trans-sialidase. As previously described, immunization of BALB/c mice with p154/13 elicited humoral, cell-mediated and protective immune responses against T. cruzi infection. In this study we describe that both P. acnes and its soluble polysaccharide fraction have the ability to modulate the immune response elicited by p154/13. Treatment with these adjuvants enhanced specific trans-sialidase Th1 immune response, as revealed by a lower IgG1/IgG2a ratio and stronger in vitro IFN-gamma synthesis by CD4+ T cells. The most important fact was that treatment with P. acnes or its soluble polysaccharide fraction in the presence of p154/13 significantly reduced the peak of parasitemia observed 7 to 8 days after T. cruzi challenge. These data suggest that P. acnes or its soluble polysaccharide fraction may improve the protective potential of a DNA vaccine against experimental T. cruzi infection.

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Citations

Oct 30, 2008·Immunology and Cell Biology·Adriana CuellarConcepción Puerta
Mar 15, 2014·PLoS Neglected Tropical Diseases·Josie Haydée Lima FerreiraClara Lúcia Barbiéri
Nov 19, 2008·Immunology Letters·Carla Cristina SquaiellaIeda Maria Longo-Maugéri
Dec 7, 2010·Scandinavian Journal of Immunology·T U MaioliA M C Faria
May 6, 2016·Frontiers in Immunology·Ana F PopiMario Mariano
Apr 18, 2015·Parasites & Vectors·Minerva Arce-FonsecaOlivia Rodríguez-Morales
Feb 23, 2018·Frontiers in Immunology·Daniela TeixeiraIeda Maria Longo-Maugéri
Jun 23, 2009·Expert Review of Vaccines·Silvia Inés CazorlaEmilio Luis Malchiodi
Jan 29, 2019·Frontiers in Molecular Neuroscience·Gabriela da Paz SilveiraIeda Maria Longo-Maugéri
Mar 21, 2020·Frontiers in Immunology·Danielle A M da SilvaClara L Barbiéri
May 31, 2020·Journal of Medical Virology·Beniamino PalmieriClaudio Cermelli
Feb 1, 2019·Annals of Dermatology·Min Ji KimWeon Ju Lee

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