Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a HIV-1 Gag DNA vaccine: different effects on cellular and humoral immunity.

Vaccine
Sumita GangulyRama Rao Amara

Abstract

Plasmid DNA immunizations induce low levels but a broad spectrum of cellular and humoral immune responses. Here, we investigate the potential of co-stimulation through 4-1BB as an adjuvant for a HIV-1 DNA vaccine in mice. We designed plasmid DNAs expressing either the membrane bound or soluble form of 4-1BBL, and compared with the agonistic anti-4-1BB Ab for their ability to adjuvant the Gag DNA vaccine. Both, anti-4-1BB agonistic Ab as well as 4-1BBL DNA enhanced the Gag-specific cellular immune responses. However, in complete contrast to the agonistic Ab that suppressed humoral immunity to Gag, 4-1BBL DNA adjuvanted vaccines enhanced Gag-specific IgG responses. Importantly, the expression of Gag and 4-1BBL from the same plasmid was critical for the adjuvant activity. Collectively, our data suggest that for a HIV-1 vaccine where both antigen-specific cellular and humoral immunity are desirable, 4-1BBL expressed by a DNA vaccine is a superior adjuvant than anti-4-1BB agonistic Ab.

References

Nov 25, 1998·Seminars in Immunology·D S Vinay, B S Kwon
May 22, 2002·Nature Immunology·E Scott HalsteadPeter D Katsikis
Apr 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lara MyersAnthony T Vella
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jacob BukczynskiTania H Watts
Sep 28, 2004·Nature Medicine·Su K SeoByoung S Kwon
Mar 18, 2005·Annual Review of Immunology·Tania H Watts
Jan 28, 2006·Journal of Virology·Geoffrey W StoneRichard S Kornbluth

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Citations

Oct 15, 2011·Journal of Industrial Microbiology & Biotechnology·Shuzhen WangYijun Chen
Aug 21, 2013·Immunological Reviews·Michael E WortzmanTania H Watts
Sep 10, 2014·Cellular & Molecular Immunology·Khalidur Rahman, Smita S Iyer
Oct 12, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Douglas C MacdonaldWilliam C Rosenberg
Oct 21, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Venkateswarlu ChamchaRama Rao Amara

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