Administration of estradiol-3-benzoate down-regulates the expression of testicular steroidogenic enzyme genes for testosterone production in the adult rat

The Journal of Veterinary Medical Science
Motoharu SakaueSeiichiroh Ohsako

Abstract

ABSTRACT. To study the role of estrogen in the testes, testosterone and testicular steroidogenic enzyme mRNA levels were investigated in male Sprague-Dawley rats 24 hr after intramuscular administration of a single dose of estradiol-3-benzoate (EB). EB administration resulted in a greater decrease in intra-testicular and serum testosterone in 10-week-old rats than in 3- or 5-week-old rats. A dose of 2 microg EB/kg had the lowest observed effect. The level of serum luteinizing hormone (LH) was unchanged at any dose. Semiquantitative RT-PCR analysis revealed that, of the four major testicular steroidogenic enzymes, mRNA levels of cytochrome P450 side-chain cleavage and 17beta-hydroxysteroid dehydrogenase type-III were significantly reduced, and mRNA levels of cytochrome P450 17alpha-hydroxylase/ C17-20 lyase (P450c17) were reduced severely and significantly, by EB administration. However, the level of 3beta-hydroxysteroid dehydrogenase type-I mRNA was not changed. In addition, the P450c17 mRNA level in EB-treated rats was much lower than that in the testes of hypophysectomized rats, with the level in the latter being equal to that in control rats. LH is secreted into blood periodically, the effects of estrogen on the LH secretion...Continue Reading

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Citations

Apr 23, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ravindranath Reddy GilibiliStelvio M Bandiera
Dec 13, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fuminori TaniguchiKenneth S Korach
Dec 15, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R BadrinarayananK Balasubramanian
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Jul 23, 2013·Clinical and Experimental Reproductive Medicine·Heejun ChiSungil Roh
Aug 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cherkaouia KibalyAyikoe G Mensah-Nyagan
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Dec 17, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Grace S LeungThomas K H Chang

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