Administration of FGF-2 to the heart stimulates connexin-43 phosphorylation at protein kinase C target sites

Cell Communication & Adhesion
Wattamon SrisakuldeeElissavet Kardami

Abstract

Fibroblast growth factor-2 (FGF-2) confers acute, preconditioning-like cardiac resistance to ischemic injury in a protein kinase C (PKC)-dependent fashion. One of the downstream targets of PKC is the gap junction protein connexin-43 (Cx43). We thus examined the effects of FGF-2 on Cx43 phosphorylation at specific PKC sites in the adult heart. Rat hearts perfused ex vivo for 20 min with an FGF-2-containing solution displayed increased levels of phosphorylated 44-45 kDa Cx43, assessed by western blotting. In addition, FGF-2 significantly upregulated phosphorylation of the PKC target serines 262 and 368 on Cx43 at intercalated disks, assessed using phosphospecific antibodies in immunolocalization and western blotting assays. Our data show that FGF-2, administered by perfusion, can alter the phosphorylation status of Cx43 at cardiomyocyte intercalated disks, and suggest a link between phosphorylation of Cx43 at specific PKC sites and FGF-2 cardioprotection.

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Citations

May 9, 2009·Cardiovascular Research·Wattamon SrisakuldeeElissavet Kardami
Mar 27, 2010·BMC Biochemistry·Corinne NigerJoseph P Stains
Jun 7, 2014·Canadian Journal of Physiology and Pharmacology·Srikanth GivvimaniSuresh C Tyagi
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Siyun LiaoJo El J Schultz
Nov 23, 2013·Journal of Molecular and Cellular Cardiology·Monte S WillisCam Patterson
Jul 19, 2011·Biochimica Et Biophysica Acta·Maya M JeyaramanElissavet Kardami
Aug 9, 2011·Biochimica Et Biophysica Acta·Lucrecia Márquez-RosadoPaul D Lampe
Oct 12, 2016·Bone·Christian Faul
May 23, 2007·Heart Failure Reviews·Elissavet KardamiPeter A Cattini
May 11, 2011·Molecular and Cellular Biochemistry·Sarah K JimenezPeter A Cattini
Mar 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Stacey L HouseJo El J Schultz
Dec 1, 2017·Cell and Tissue Research·Ajaz Ahmad WazaBashir A Ganai
Oct 9, 2020·Growth Factors·Maria Rosaria De PascaleClaudio Napoli
Apr 28, 2007·Progress in Biophysics and Molecular Biology·Elissavet KardamiDavid C Spray
Jul 10, 2013·European Journal of Pharmacology·Ada PopoloAldo Pinto

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