Administration of human peripheral blood-derived CD133+ cells accelerates functional recovery in a rat spinal cord injury model

Spine
H SasakiM Ochi

Abstract

Magnetically isolated, peripheral blood-derived CD133+ cells were used as the therapeutic agent of spinal cord injury (SCI). A rat model was used to investigate the hypothesis that the cell therapy using this clinically accessible cell fraction could be an attractive option for injured spinal cord. Given the capacity for the peripheral blood-derived CD133+ cells in vivo to produce neurogenesis via vasculogenesis as the feasible candidate for SCI in the clinical setting, the focus of the experiment was to investigate whether the cells could contribute to histologic and functional recovery of SCI after transplantation. No evidence for peripheral blood-derived CD133+ cells application to SCI and no experimental studies showed functional recovery from SCI using this cell fraction have been published. Contusion SCI was induced by placing a 25-g rod onto the spinal cord for 90 seconds in athymic nude rats. CD133+ cells or phosphate-buffered saline was administered intravenously immediately after SCI. The animals were analyzed at specific times after transplantation by several methods to examine histologic vasculogenesis and neurogenesis and to confirm functional recovery from SCI. After cell transplantation, intrinsic angiogenesis an...Continue Reading

References

Jul 1, 1989·Electroencephalography and Clinical Neurophysiology·M G FehlingsR D Linden
Feb 1, 1995·Journal of Neurotrauma·D M BassoJ C Bresnahan
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Sep 17, 2003·Cardiovascular Radiation Medicine·Atsuhiko KawamotoDouglas W Losordo
May 18, 2004·Experimental Neurology·Jeffrey M Rosenstein, Janette M Krum
Apr 1, 2006·Medical Molecular Morphology·Takato UenoMichio Sata
May 2, 2006·Archivum Immunologiae Et Therapiae Experimentalis·Magda KuciaMariusz Z Ratajczak

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Citations

Mar 2, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Janelle M FassbenderTheo Hagg
Jan 10, 2013·Tissue Engineering. Part C, Methods·Shingo OhkawaMitsuo Ochi
Nov 13, 2010·International Archives of Medicine·Thomas E IchimNeil H Riordan
May 14, 2014·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Mitsuo OchiHussein El Mahmoud
Mar 1, 2014·The Journal of Sexual Medicine·Katsutoshi MiyamotoAkio Matsubara
Jun 27, 2014·The Journal of Spinal Cord Medicine·Edyta PaczkowskaBogusław Machaliński
Mar 19, 2013·Journal of the Neurological Sciences·Naosuke KameiTakayuki Asahara
Feb 19, 2013·Journal of Neurology, Neurosurgery, and Psychiatry·Claire M RiceNeil J Scolding
May 18, 2021·Frontiers in Physiology·Chun YaoBin Yu

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