Administration of M. leprae Hsp65 interferes with the murine lupus progression.

PloS One
Eliana Blini MarengoOsvaldo Augusto Sant'anna

Abstract

The heat shock protein [Hsp] family guides several steps during protein synthesis, are abundant in prokaryotic and eukaryotic cells, and are highly conserved during evolution. The Hsp60 family is involved in assembly and transport of proteins, and is expressed at very high levels during autoimmunity or autoinflammatory phenomena. Here, the pathophysiological role of the wild type [WT] and the point mutated K(409)A recombinant Hsp65 of M. leprae in an animal model of Systemic Lupus Erythematosus [SLE] was evaluated in vivo using the genetically homogeneous [NZBxNZW]F(1) mice. Anti-DNA and anti-Hsp65 antibodies responsiveness was individually measured during the animal's life span, and the mean survival time [MST] was determined. The treatment with WT abbreviates the MST in 46%, when compared to non-treated mice [p<0.001]. An increase in the IgG2a/IgG1 anti-DNA antibodies ratio was also observed in animals injected with the WT Hsp65. Incubation of BALB/c macrophages with F(1) serum from WT treated mice resulted in acute cell necrosis; treatment of these cells with serum from K(409)A treated mice did not cause any toxic effect. Moreover, the involvement of WT correlates with age and is dose-dependent. Our data suggest that Hsp65 m...Continue Reading

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Citations

Mar 29, 2014·Immunity & Ageing : I & a·Estevam José BaldonOsvaldo Augusto Sant'anna
Nov 26, 2009·PloS One·Eliana B MarengoOsvaldo Augusto Sant'Anna
Oct 1, 2011·PloS One·Eliana B MarengoOsvaldo Augusto Sant'Anna
Dec 29, 2009·Toxicon : Official Journal of the International Society on Toxinology·Maria de Fátima D FurtadoOsvaldo Augusto Sant'Anna
Sep 12, 2012·Autoimmune Diseases·Alessandra Gonçalves CommodaroLuiz Vicente Rizzo
Feb 18, 2015·The Journal of Antimicrobial Chemotherapy·Rodrigo F RodriguesCelio L Silva
Mar 1, 2010·Einstein·Alessandra Gonçalves CommodaroLuiz Vicente Rizzo
Jan 26, 2021·Journal of Translational Autoimmunity·Michael Battaglia, Lee Ann Garrett-Sinha

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Methods Mentioned

BETA
ELISA
light microscopy
enzyme-linked immunosorbent assay
flow cytometry

Software Mentioned

CellQuest

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