Sep 15, 2020

Adoptive cell therapy of triple negative breast cancer with redirected cytokine-induced killer cells

Oncoimmunology
Roberta SommaggioAntonio Rosato

Abstract

Cytokine-Induced Killer (CIK) cells share several functional and phenotypical properties of both T and natural killer (NK) cells. They represent an attractive approach for cell-based immunotherapy, as they do not require antigen-specific priming for tumor cell recognition, and can be rapidly expanded in vitro. Their relevant expression of FcγRIIIa (CD16a) can be exploited in combination with clinical-grade monoclonal antibodies (mAbs) to redirect their lytic activity in an antigen-specific manner. Here, we report the efficacy of this combined approach against triple negative breast cancer (TNBC), an aggressive tumor that still requires therapeutic options. Different primitive and metastatic TNBC cancer mouse models were established in NSG mice, either by implanting patient-derived TNBC samples or injecting MDA-MB-231 cells orthotopically or intravenously. The combined treatment consisted in the repeated intratumoral or intravenous injection of CIK cells and cetuximab. Tumor growth and metastasis were monitored by bioluminescence or immunohistochemistry, and survival was recorded. CIK cells plus cetuximab significantly restrained primitive tumor growth in mice, either in patient-derived tumor xenografts or MDA-MB-231 cell line m...Continue Reading

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Mentioned in this Paper

Immunohistochemistry
Protein Expression
Patient Derived Xenograft
Peripheral
NKG2D Receptor Binding
Cytokine
Bioluminescent Measurements
ErbB-2 Receptor
Fetus
ISO-1 cpd

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