Adoptive transfer of mesenchymal stromal cells accelerates intestinal epithelium recovery of irradiated mice in an interleukin-6-dependent manner

Cytotherapy
Moïra FrançoisJacques Galipeau

Abstract

Apoptosis of radiosensitive cells in the bone marrow and gut is a serious, at times life-threatening, complication arising from radiation exposure. We investigated whether adoptive transfer of allogeneic bone marrow-derived mesenchymal stromal cells (MSC) could exert cytoprotective and life-sparing effects in a mouse model of sublethal total body irradiation (TBI). We demonstrated that a single intraperitoneal injection of C57Bl/6 MSC given to major histocompatibility complex (MHC)-mismatched Balb/c mice within 24 h of sublethal TBI significantly reduced mortality in a dose-dependent manner. Histologic analysis and Ki67 immunostaining of jejunum sections collected 3 and 6 days post-TBI indicated that MSC protected the gastrointestinal epithelium from TBI-induced damage and significantly accelerated recovery of the gut by stimulating proliferation of the crypt cell pool. Using interleukin-6(-/-) (IL-6) MSC, we demonstrated that IL-6 expressed by MSC played a role in gastrointestinal epithelium regeneration. Our results suggest that allogeneic MHC-mismatched MSC may be exploited to reduce gastrointestinal complications and mortality arising from ionizing radiation exposure.

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 4, 2005·Diseases of the Colon and Rectum·Damián García-OlmoJosé Antonio Rodríguez-Montes
Aug 2, 2007·Transplantation·Aisha NasefLoïc Fouillard
Nov 21, 2007·Seminars in Cell & Developmental Biology·Gary BrookeKerry Atkinson
May 14, 2008·Experimental Hematology·Ben J Jones, Steven J McTaggart
May 7, 2010·Health Physics·Marc BenderitterJean Jacques Lataillade

❮ Previous
Next ❯

Citations

Oct 24, 2013·Antioxidants & Redox Signaling·Marc BenderitterCharles L Limoli
Jul 15, 2015·Cancer Letters·Nils H NicolayPeter E Huber
May 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Victoria JefferyAnastasia Sobolewski
Jan 10, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Raghavan ChinnaduraiJacques Galipeau
May 7, 2015·World Journal of Gastroenterology : WJG·Raghavan ChinnaduraiJacques Galipeau
Sep 19, 2020·Current Stem Cell Reports·Raghavan ChinnaduraiChristian M Capitini
Oct 5, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Naz ChaudaryMichael Milosevic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.